L0881

Lectin from Arachis hypogaea (peanut)

lyophilized powder, Affinity-purified

• Synonym(s): PNA, Peanut agglutinin
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• form: lyophilized powder
• Quality Level: 200
• potency: <0.1 μg per mL agglutination activity
• quality: Affinity-purified
• impurities: salt, free
• storage temp.: −20°C

Description

General description

Lectin from Arachis hypogaea (peanut) is a homotetrameric protein of 110 kDa.

Application

Lectin from Arachis hypogaea (peanut) has been used:

• to detect Thomson-Friedenreich antigen
• as a component in PBS to study its effects on cell viability
• to study its ability to block the binding site

Biochem/physiol Actions

Lectin from Arachis hypogaea (peanut) possess specificity towards β -D-Gal(1-3)-D-galNAc residue of Thomson − Friedenreich antigen. It is the most widely used diagnostic and prognostic marker for many squamous cell carcinomas.

PNA does not agglutinate normal human erythrocytes, but strongly agglutinates neuraminidase treated erythrocytes. PNA has potent anti-T activity similar to the anti-T antibody in human sera. The lectin can be used to distinguish between human lymphocyte subsets.

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of neuraminidase treated human blood group O erythrocytes after 1 hr incubation at 25 °C.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves