M2634

Malic Dehydrogenase from porcine heart

buffered aqueous glycerol solution, 600-1000 units/mg protein (biuret)

• Synonym(s): L-Malate: NAD⁺ oxidoreductase, MDH, Malate Dehydrogenase
• EC Number: 1.1.1.37 (BRENDA, IUBMB)
• MDL number: MFCD00131567
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• form: buffered aqueous glycerol solution
• Quality Level: 200
• specific activity: 600-1000 units/mg protein (biuret)
• cation traces: NH₄⁺: ≤10 μg/mg protein
• foreign activity: Glutamic-Oxalacetic Transaminase ≤0.01%; Glutamic-Pyruvic Transaminase ≤0.01%
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Malic dehydrogenase is a mitochondrial isozyme and an important catalyst in the tricarboxylic acid cycle.

Mitochondrial Isozyme

Application

Malic dehydrogenase has been used in a study to assess the comparative molluscicidal action of Ginko biloba extract on snail hosts. It has also been used in a study to investigate the effect of early feed restriction on metabolic programming and compensatory growth in broiler chickens.

Biochem/physiol Actions

Eukaryotic cells contain two different isozymes of malate dehydrogenase: mitochondrial (m-MDH) and soluble or cytoplasmic (s-MDH). This product consists of the mitochondrial form.
The enzyme catalyzes the following reaction:Oxaloacetate + β-NADH → L-Malate + β-NAD

Malic Dehydrogenase can be reversibly inactivated by treatment with pyridoxal 5′-phosphate at 25 °C.

Unit Definition

One unit will convert 1.0 μmole of oxalacetate and β-NADH to _L-malate and β-NAD per min at pH 7.5 at 25 °C.

Physical form

Solution in 50% glycerol containing 0.05 M potassium phosphate buffer, pH 7.5

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves