M8284

Maltose Phosphorylase from Enterococcus sp.

recombinant, expressed in E. coli, lyophilized powder

• Synonym(s): Maltose:orthophosphate 1-β-D-Glucosyltransferase
• CAS Number: 9030-19-7
• EC Number: 2.4.1.8 (BRENDA, IUBMB)
• MDL number: MFCD03457426
• UNSPSC Code: 12352204
• PubChem Substance ID: 24897239
• NACRES: NA.54

Properties

• recombinant: expressed in E. coli
• Quality Level: 200
• form: lyophilized powder
• specific activity: ≥9 units/mg solid
• mol wt: 90 kDa by SDS-PAGE
• storage temp.: −20°C
• SMILES string: OC(=O)CCCCCCCCCCCCC
• InChI: 1S/C14H28O2/c1-2-3-4-5-6-7-8-9-10-11-12-13-14(15)16/h2-13H2,1H3,(H,15,16)
• InChI key: TUNFSRHWOTWDNC-UHFFFAOYSA-N

Description

General description

Maltose phosphorylase (MP) is a dimeric enzyme. This enzyme is classified under family 65 of the glycoside hydrolases. It is a member of the disaccharide phosphorylase family.

Application

Maltose Phosphorylase from Enterococcus sp. has been used as a component in coupled assay and ATPase activity assay to study its effects on ATPase activity, on the 90 kDa heat shock proteins (Hsp90) ATPase reaction and on background absorbance on the production of resorufin.

Maltose phosphorylase from Enterococcus has been used in a study to describe a new pathway for maltose utilization in lactic acid bacteria. It has also been used in a study to describe the transfer of glucosyl moiety of maltose to acceptors with alcoholic OH groups.

Biochem/physiol Actions

Enzymatically converts maltose to D-Glucose.

Maltose phosphorylase (MP) is a dimeric enzyme that catalyzes maltose and inorganic phosphate into β-D-glucose-1-phosphate and glucose.

Maltose phosphorylase not only transfers glucosyl moieties from maltose to other sugars but has been shown to also use phenolic compounds such as salicyl alcohol as acceptors.

Unit Definition

One unit will produce 1.0 μmole of D-Glucose from maltose per minute at pH 7.0 at 30°C.

Other Notes

Contains lactose.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable