P1261

Phosphorylase a from rabbit muscle

lyophilized powder, 20-30 units/mg protein

• Synonym(s): 1,4-α-D-Glucan:orthophosphate α-D-glucosyltransferase
• CAS Number: 9032-10-4
• EC Number: 2.4.1.1 (BRENDA, IUBMB)
• EC Number: 232-878-8
• MDL number: MFCD00167428
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: rabbit muscle
• Quality Level: 200
• form: lyophilized powder
• specific activity: 20-30 units/mg protein
• purified by: 2× crystallization
• composition: Protein, 30-60% E1%/280
• foreign activity: AMP-phosphatase, ATP-phosphatase, debrancher enzyme and phosphorylase phosphatase ≤0.1%; phosphoglucomutase ≤0.25%; phosphorylase b ≤30%
• storage temp.: −20°C

Description

General description

Phosphorylase A is the active form of glycogen phosphorylase which converts glycogen and orthophosphate (Pi) to glucose 1-phoshate (G-1-P).

Application

Phosphorylase a from rabbit muscle has been used to mix with glycogen for the preparation of limit dextrin. It has also been used to probe the array in oligosaccharide microarray analysis of phosphorylase activity.

Phosphorylase from rabbit muscle has been used in a study to assess the molecular mechanisms of oleanolic acid. It has also been used in a study to describe the conversion of phosphorylase B to A, through a conversion enzyme in the presence of ³²P-ATP.

Biochem/physiol Actions

Dimeric phosphorylase b is converted to the more active tetramer, phosphorylase a, by the action of phosphorylase kinase.

Phosphorylase A can be inhibited by these compounds: Polychlorinated biphenyls, polychlorinated biphenylols and polybrominated biphenyls.

Phosphorylase participates in glycogenolysis. Deficiency of phosphorylase leads to several glycogen storage diseases with hepatomegaly.

Unit Definition

One unit will form 1.0 μmole of α-D-glucose 1-phosphate from glycogen and orthophosphate per min at pH 6.8 at 30 °C, measured in a system containing phosphoglucomutase, NADP, and glucose-6-phosphate dehydrogenase. (One μmolar unit is equivalent to ~45 Cori units.)

Physical form

Lyophilized powder containing β-glycerophosphate and EDTA

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves