N3643

NZCYM Broth

Powder microbial growth medium

• Synonym(s): Broth medium
• UNSPSC Code: 41106200
• NACRES: NA.85

Properties

• grade: Molecular Biology
• Quality Level: 200
• sterility: non-sterile
• form: powder
• composition: Casamino Acids, 1 g/L ; MgSO₄, 0.98 g/L ; NaCL, 5 g/L ; Tryptone, 10 g/L ; Yeast extract, 5 g/L
• technique(s): microbiological culture: suitable
• pH: 6.8-7.2(2.2% solution)
• application(s): food and beverages; microbiology
• storage temp.: room temp
• suitability: nonselective for Escherichia coli; nonselective for coliforms

Description

General description

NZCYM is a microbial growth medium used for the cultivation of E. coli and lambda bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.

Application

NZCYM Broth has been used for non-selective cultivation of lambda, MS2, T7, CLP-P3, Phi29, and Goe1 bacteriophages, and E. coli strains for cloning, DNA plasmid production, and production of recombinant proteins. It is also suitable for selective cultivation when appropriate antibiotics are added.

Features and Benefits

NZCYM powder provides:

• Standard formulation
• Convenient package sizes
• A budget-friendly alternative to liquid

Reconstitution

Stir to suspend 22 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves