T5574

Terrific Broth

Liquid microbial growth medium

• Synonym(s): TB medium, Tartoff – Hobbs medium
• UNSPSC Code: 41106200
• NACRES: NA.85

Properties

• grade: Molecular Biology
• Quality Level: 500
• description: Liquid Medium
• sterility: sterile; 0.2 μm filtered
• form: liquid
• technique(s): microbiological culture: suitable
• application(s): microbiology
• storage temp.: room temp
• suitability: nonselective for Escherichia coli; nonselective for coliforms

Description

General description

Terrific Broth is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains glycerol, peptides, amino acids, water-soluble vitamins, and carbohydrates.

Application

Terrific Broth has been used for non-selective cultivation of E. coli strains for cloning, high-yield DNA plasmid production, and high-yield production of recombinant proteins. citation It is also suitable for selective cultivation when appropriate antibiotics are added.

Features and Benefits

Terrific Broth liquid provides:

• Filter-sterilized, ready-to-use format
• Convenient package size
• Standard formulation

Components

23.6g/L Yeast Extract
11.8g/L Tryptone
9.4g/L K₂HPO₄
2.2g/L KH₂PO₄
4ml/L Glycerol

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves