N9914

Polynucleotide phosphorylase from Synechocystis sp.

recombinant, expressed in E. coli

• Synonym(s): PNPase, Polyribonucleotide Nucleotidyltransferase
• EC Number: 2.7.7.8 (BRENDA, IUBMB)
• MDL number: MFCD00131992
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: bacterial (Synechocystis sp.)
• Quality Level: 200
• recombinant: expressed in E. coli
• description: Histidine tagged
• Assay: 90% (SDS-PAGE)
• form: solution
• specific activity: ≥500 units/mg protein
• mol wt: 85 kDa
• technique(s): cell based assay: suitable
• suitability: suitable for molecular biology
• application(s): cell analysis
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

Polynuclotide phosphorlyase in spinach chloroplasts acts as a exonuclease and a poly(A) polymerase.

Application

Polynucleotide phosphorylase has been used in a study to discover that a major function of PNPase is the synthesis of CDP. It has also been used in a study to investigate the enzyme responsible for RNA 3′-tail synthesis in S. coelicolor.

Biochem/physiol Actions

Polynucleotide phosphorylase (PNPase) is a bifunctional enzyme with a phosphorolytic 3′ to 5′ exoribonuclease activity and a 3′-terminal oligonucleotide polymerase activity.

Polynucleotide phosphorylase localizes to the intermembrane space of mitochondria and has a critical function in regulating mitochondrial homeostasis in human cells.

Unit Definition

One unit will polymerize 1.0 μmole of ADP, releasing 1.0 μmole of inorganic phosphate in 15 minutes, at pH 9.1 at 37 °C.

Supplied as a solution in 20 mM Hepes buffer pH 7.9, 0.1 mM EDTA, 2 mM DTT, 12.5 mM MgCl₂, 60 mM KCl, 20% (w/v) Glycerol

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable