P1644

Percoll^®

pH 8.5-9.5 (20 °C)

• Synonym(s): Percoll Density Gradient Media
• MDL number: MFCD00131830
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• sterility: aseptically filled
• Quality Level: 200
• form: colloidal ((Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)) )
• technique(s): cell culture | mammalian: suitable
• color: clear colorless to faint yellow
• pH: 8.5-9.5 (20 °C)
• application(s): hematology; histology
• storage temp.: 2-8°C

Description

General description

Percoll^® is a classic medium for density gradient centrifugation of cells, viruses, and subcellular particles. Percoll consists of colloidal silica particles of 15–30 nm diameter (23% w/w in water), which have been coated with polyvinylpyrrolidone (PVP). The PVP coating renders the product completely non-toxic and ideal for use with biological materials.

Application

Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). It is used in cell separation and organelle isolation.

Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). It is used to establish non-toxic low viscosity, low osmolarity density gradients that can be used to isolate cells, organelles and viruses. It has been used to separate inner and outer membrane vesicles from E. coli. Percoll is isosmotic throughout the gradient, non-toxic, and is easily removed from the purified materials.

Percoll^® has been used in sperm preparation to study the energy requirements of bovine embryos.

Legal Information

Percoll is a registered trademark of Cytiva

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves