P7828

Percoll^®

suitable for plant cell culture

• Synonym(s): density gradient medium
• MDL number: MFCD00131830
• UNSPSC Code: 10171502
• NACRES: NA.72

Properties

• sterility: aseptically filled
• Quality Level: 200
• form: colloidal (Colloidal solution of silica coated with polyvinylpyrrolidone (PVP))
• technique(s): cell culture | plant: suitable
• pH: 8.9 (20 °C)
• application(s): agriculture
• storage temp.: 2-8°C

Description

Application

Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). It is used in cell separation and organelle isolation.

Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). It is used to establish non-toxic low viscosity, low osmolarity density gradients that can be used to isolate cells, organelles and viruses. It has been used to separate inner and outer membrane vesicles from E. coli. Percoll is isosmotic throughout the gradient, non-toxic, and is easily removed from the purified materials.

Percoll is used in plant research for protoplast and organelle (chloroplast, mitochondria) separation.

Percoll^® has been used in the density gradient centrifugation isolation of intact chloroplasts from green alga, decidual stromal cells and distinct populations of rooster sperm.

Legal Information

Percoll is a registered trademark of Cytiva

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves