P4762
Papain from papaya latex
lyophilized powder, ≥10 units/mg protein
Synonym(s):
Papainase
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About This Item
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biological source
papaya (latex)
form
lyophilized powder
specific activity
≥10 units/mg protein
mol wt
23.4 kDa
technique(s)
cell culture | mammalian: suitable
single cell analysis: suitable
application(s)
diagnostic assay manufacturing
storage temp.
−20°C
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General description
Papain is a cysteine protease, found in Carica papaya. It is composed of a single polypeptide chain containing three disulfide bridges and a sulfhydryl group.
Application
Papain from papaya latex has been used:
- to digest carotid artery smooth muscle cells
- in dissecting solutions
- to produce Fab fragments of antibodies
- for cell dissociation since it has been shown to be more effective and less damaging with certain tissues
- in the enzyme cocktail for dissociating human fetal retinal tissue
- to digest chondrocytes/scaffold cultures for total DNA quantification for cell proliferation
- as a supplement in neurobasal medium to produce retinal cell suspensions
Used to produce Fab fragments of antibodies. Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues.
Biochem/physiol Actions
A cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
pH optimum 6.0-7.0
Also hydrolyzes esters and amides.
pH optimum 6.0-7.0
Also hydrolyzes esters and amides.
Papain with wide specificity, cleaves peptide bonds of basic amino acids, leucine or glycine. It also hydrolyzes esters and amides. Papain is considered to digest protein substrates more broadly than the pancreatic proteases. Papain is commonly used in cell isolation procedures as it is highly efficient and less harmful than other proteases on certain tissues. The use of papain is known to yield more viable smooth muscle cells, retaining the cell′s sensitivity to stimulants. Papain papaya latex has antifungal activity against C. albicans and is a cysteine protease.
- The pH optimum is 6.0-7.0
- Papain hydrolyzes esters and amides
Packaging
Package size based on protein content
Unit Definition
One unit will hydrolyze 1.0 micromole of N-alpha-benzoyl-L-arginine ethyl ester (BAEE) per minute at pH 6.2 at 25 deg C.
Physical form
Lyophilized powder containing sodium acetate
Preparation Note
2× Crystallized
Other Notes
View more information on papain at www.sigma-aldrich.com/enzymeexplorer.
inhibitor
Product No.
Description
Pricing
substrate
Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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