P5860

Protease from Bacillus sp.

liquid, ≥8 U/g

• Synonym(s): Esperase^® 8.0L
• EC Number: 232-752-2
• MDL number: MFCD00132092
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• form: liquid
• Quality Level: 200
• specific activity: ≥8 U/g
• mol wt: 20--30 kDa
• storage temp.: 2-8°C

Description

Application

Protease is an enzyme used to break down proteins by hydrolyzing peptide bonds. Product P5860 is secreted from Bacillus sp. It is a serine-type protease characterized by excellent perfomance at elevated temperature and pH. Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics. The enzyme has been used to test UV radiation as an environmental friendly, non-thermal technology for sterilizing enzymes used in the food industry.

Biochem/physiol Actions

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate. Proteases, secreted from Bacillus sp., typically have molecular weights ranging from 20,000 to 30,000. They are typcially stabilized by Ca²⁺ and have high isoelectric points.

Legal Information

A product of Novozyme corp.

Esperase is a registered trademark of Novozymes Corp.

Safety Information

• Pictograms: GHS08,GHS05,GHS07,GHS09
• Signal Word: Danger
• Hazard Statements: H302,H315,H318,H334,H335,H410
• Precautionary Statements: P261 - P273 - P280 - P305 + P351 + P338 - P342 + P311
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves