S7632

N-Succinyl-Ala-Ala-Val-Ala p-nitroanilide

protease substrate

• Empirical Formula (Hill Notation): C₂₄H₃₄N₆O₉
• CAS Number: 108322-03-8
• Molecular Weight: 550.56
• MDL number: MFCD00133618
• UNSPSC Code: 12352204
• PubChem Substance ID: 24899748
• NACRES: NA.32

Properties

• Quality Level: 200
• Assay: ≥98% (TLC)
• form: powder
• solubility: DMF: 10 mg/mL, clear, colorless
• UniProt accession no.: P57774
• storage temp.: −20°C
• SMILES string: CC(C)C(NC(=O)C(C)NC(=O)C(C)NC(=O)CCC(O)=O)C(=O)NC(C)C(=O)Nc1ccc(cc1)N(=O)=O
• InChI: 1S/C24H34N6O9/c1-12(2)20(24(37)27-14(4)22(35)28-16-6-8-17(9-7-16)30(38)39)29-23(36)15(5)26-21(34)13(3)25-18(31)10-11-19(32)33/h6-9,12-15,20H,10-11H2,1-5H3,(H,25,31)(H,26,34)(H,27,37)(H,28,35)(H,29,36)(H,32,33)
• InChI key: IYHHIHIBYVIDMJ-UHFFFAOYSA-N
• Gene Information: mouse ... Npy(109648)

Description

General description

N-Succinyl-Ala-Ala-Val-Ala p-nitroanilide is a chromogenic and a synthetic peptide substrate for proteases, elastase and subtilisin.

Application

N-Succinyl-Ala-Ala-Val-Ala p-nitroanilide has been used as a synthetic peptide substrate for:

• Bacillus pumilus alkaline protease (DHAP)
• mesophilic subtilisin Carlsberg (sC) to assess the hydrolytic activity of wild-type and mutant sC
• Pseudoalteromonas arctica cold-active protease (Pro21717)

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves