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S7632

Sigma-Aldrich

N-Succinyl-Ala-Ala-Val-Ala p-nitroanilide

protease substrate

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About This Item

Empirical Formula (Hill Notation):
C24H34N6O9
CAS Number:
Molecular Weight:
550.56
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.32

Quality Level

Assay

≥98% (TLC)

form

powder

solubility

DMF: 10 mg/mL, clear, colorless

UniProt accession no.

storage temp.

−20°C

SMILES string

CC(C)C(NC(=O)C(C)NC(=O)C(C)NC(=O)CCC(O)=O)C(=O)NC(C)C(=O)Nc1ccc(cc1)N(=O)=O

InChI

1S/C24H34N6O9/c1-12(2)20(24(37)27-14(4)22(35)28-16-6-8-17(9-7-16)30(38)39)29-23(36)15(5)26-21(34)13(3)25-18(31)10-11-19(32)33/h6-9,12-15,20H,10-11H2,1-5H3,(H,25,31)(H,26,34)(H,27,37)(H,28,35)(H,29,36)(H,32,33)

InChI key

IYHHIHIBYVIDMJ-UHFFFAOYSA-N

Gene Information

mouse ... Npy(109648)

General description

N-Succinyl-Ala-Ala-Val-Ala p-nitroanilide is a chromogenic and a synthetic peptide substrate for proteases, elastase and subtilisin.

Application

N-Succinyl-Ala-Ala-Val-Ala p-nitroanilide has been used as a synthetic peptide substrate for:
  • Bacillus pumilus alkaline protease (DHAP)
  • mesophilic subtilisin Carlsberg (sC) to assess the hydrolytic activity of wild-type and mutant sC
  • Pseudoalteromonas arctica cold-active protease (Pro21717)

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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