E7885

Elastase from porcine pancreas

lyophilized powder, suitable for cell culture

• Synonym(s): Elastase from hog pancreas, Pancreatopeptidase E
• CAS Number: 39445-21-1
• EC Number: 3.4.21.36 (BRENDA, IUBMB)
• EC Number: 254-453-6
• MDL number: MFCD00130998
• UNSPSC Code: 12352204
• NACRES: NA.75

Properties

• biological source: Porcine
• form: lyophilized powder
• specific activity: ≥4 units/mg protein
• mol wt: 25.9 kDa
• packaging: pkg of 1 μmol
• technique(s): cell culture | mammalian: suitable
• pH: 8
• suitability: suitable for
• foreign activity: Trypsin ≤50 BAEE units/mg protein
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin.

Application

Elastase from porcine pancreas is used for the following applications:

• Cell culture techniques
• ELISA techniques
• Used in assay for elastase inhibitory activity

Biochem/physiol Actions

Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.

Enzyme that degrades elastin, a matrix component of tissues involved in stretching processes. It is a serine protease with broad specificity. It cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester.

Packaging

Package size based on protein content

Unit Definition

One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25 °C.

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Danger
• Hazard Statements: H315,H319,H334,H335
• Precautionary Statements: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable