E7885
Elastase from porcine pancreas
lyophilized powder, suitable for cell culture
Synonym(s):
Elastase from hog pancreas, Pancreatopeptidase E
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About This Item
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.75
Recommended Products
biological source
Porcine
form
lyophilized powder
specific activity
≥4 units/mg protein
mol wt
25.9 kDa
packaging
pkg of 1 μmol
technique(s)
cell culture | mammalian: suitable
pH
8
suitability
suitable for
foreign activity
Trypsin ≤50 BAEE units/mg protein
shipped in
dry ice
storage temp.
−20°C
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General description
Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin.
Application
Elastase from porcine pancreas is used for the following applications:
- Cell culture techniques
- ELISA techniques
- Used in assay for elastase inhibitory activity
Biochem/physiol Actions
Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.
Enzyme that degrades elastin, a matrix component of tissues involved in stretching processes. It is a serine protease with broad specificity. It cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester.
Packaging
Package size based on protein content
Unit Definition
One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25 °C.
Application
Product No.
Description
Pricing
inhibitor
Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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P Hamet et al.
The Journal of biological chemistry, 264(21), 12364-12369 (1989-07-25)
The induction of cyclic GMP formation in target tissues, i.e. vascular smooth muscle and endothelial cells, by atrial natriuretic factor is followed by its egression into plasma and urine. Since the extracellular appearance of this cyclic nucleotide is used as
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Nihon Ishinkin Gakkai zasshi = Japanese journal of medical mycology, 48(1), 13-18 (2007-02-09)
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Protocols
Enzymatic Assay of Elastase (EC 3.4.21.36), a continuous spectrophotometric rate determination.
Chromatograms
application for HPLCOur team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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