S8633

Sphingomyelinase from Staphylococcus aureus

buffered aqueous glycerol solution, 100-300 units/mg protein (Lowry)

• Synonym(s): Sphingomyelin choline phosphohydrolase, Sphingomyelin phosphodiesterase
• CAS Number: 9031-54-3
• EC Number: 3.1.4.12 (BRENDA, IUBMB)
• MDL number: MFCD00132362
• UNSPSC Code: 12352204
• NACRES: NA.32

Properties

• form: buffered aqueous glycerol solution
• Quality Level: 200
• specific activity: 100-300 units/mg protein (Lowry)
• storage temp.: 2-8°C

Description

Application

Sphingomyelinase from Staphylococcus aureus has been used to:

• induce neurotoxicity in rat cortical cultures to study the protective effects of minocycline
• determine the concentration of sphingomyelin from serum samples
• enhance sphingomyelinase activity to study PARK9-mediated exosome biogenesis

Biochem/physiol Actions

Bacterial sphingomyelinase is active at neutral pH. When used in cell culture in vitro, it hydrolyzes the sphingomyelin on the outer leaflet of the plasma membrane and produces ceramide that is lipid-soluble. Sphingomyelinase is the key enzyme in the sphingomyelinase/ceramide pathway, which is implicated in the pathogenesis of several neurodegenerative disorders.

Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin.

Unit Definition

One unit will hydrolyze 1.0 μmol of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C.

Physical form

Solution in 50% glycerol containing 0.25 M phosphate buffer, pH 7.5

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves