SRE0118

dsDNase

recombinant, expressed in Pichia pastoris, heat-labile, Triton-free

• Synonym(s): double strand specific deoxyribonuclease
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• recombinant: expressed in Pichia pastoris
• Quality Level: 200
• specific activity: (Ca. 400 000 Kunitz Units/mg)
• feature: heat-labile
• composition: Triton-free
• packaging: pkg of 250 U
• optimum pH: 7.5
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

dsDNase is a heat-labile endonuclease that has a strong preference for double-stranded DNA. dsDNase can be heat inactivated by heat treatment at 15 min at 65˚C, or 20 min at 60˚C. The enzyme requires 1 mM DTT and pH ≥ 8 for complete inactivation.

Application

This dsDNase is a useful tool to remove contaminating DNA from PCR mastermixes.

Features and Benefits

• This nuclease has double-stand specificity, which allows specific degradation of dsDNA while leaving shorter ssDNA as primers.
• Easy inactivation by moderate heat (65°C) allows addition of DNA intended for analysis directly after removal of contaminating DNA.
• The specific activity is estimated to be 30 times higher than that of bovine DNase I.

Activity towards dsDNA is 5000-fold higher than towards ssDNA

Unit Definition

One Unit is defined as an increase in absorbance at 260 nm of 0.001 per minute, using 50 µg/ml high MW DNA in 100 mM Na-acetate pH 5.0 and 5 mM MgCl₂.

Reconstitution

In solution.

Other Notes

The dsDNase is highly active in a temperature range of 20-40°C. It needs at least 2.5 mM Mg for activity and has an optimal pH at 7.5.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable