U8501

Uridine-5′-diphosphoglucose pyrophosphorylase from baker′s yeast

Type X, lyophilized powder, ≥40 units/mg protein

• Synonym(s): UTP:α-D-glucose-1-phosphate uridylyltransferase
• CAS Number: 9026-22-6
• EC Number: 2.7.7.9 (BRENDA, IUBMB)
• EC Number: 232-811-2
• MDL number: MFCD00132561
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: bakers yeast
• Quality Level: 200
• type: Type X
• form: lyophilized powder
• specific activity: ≥40 units/mg protein
• composition: Protein, 30-60% modified Warburg-Christian
• foreign activity: UDP-glucose dehydrogenase and galactose-1-phosphate uridyltransferase ≤0.1%; inorganic pyrophosphatase ≤0.5%
• storage temp.: −20°C

Description

General description

Uridine-5′-diphosphoglucose pyrophosphorylase (UDP-glc-PPase) is ubiquitous in plants, yeast, bacteria, and mammals. This enzyme is an octamer of eight identical sub-units.

Application

Uridine-5′-diphosphoglucose pyrophosphorylase from baker′s yeast has been used:

• in the synthesis of uridine-5′-diphosphoglucose (UDP-Glc)-¹³C9
• to quantify Suc synthase (SUS) enzyme activity in rice
• to study the role of hexokinase and glycogen synthase controls the flux in frog oocytes

Biochem/physiol Actions

Uridine-5′-diphosphoglucose pyrophosphorylase (UDP-glc-PPase) participates in catalyzing the synthesis of UDP-glucose. This enzyme requires divalent cations such as Mg²⁺, Ca²⁺, Mn²⁺, Ni²⁺ for its activity.

Unit Definition

One unit will form 1.0 μmole of glucose 1-phosphate from uridine-5′-diphosphoglucose and inorganic pyrophosphate per min at pH 7.6 at 25 °C.

Physical form

Lyophilized, sulfate-free powder containing citrate buffer salt

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves