Y1251

Yeast Nitrogen Base Without Amino Acids and Ammonium Sulfate

suitable for microbiology, NutriSelect^® Basic

• UNSPSC Code: 41171606
• NACRES: NA.85

Properties

• sterility: non-sterile
• Quality Level: 200
• form: powder
• packaging: poly bottle of 1 kg; poly bottle of 500 g
• manufacturer/tradename: NutriSelect^® Basic
• solubility: water: soluble 67 mg/mL
• application(s): clinical testing; food and beverages; life science and biopharma; microbiology
• suitability: Candida spp.; Pichia spp.; Saccharomyces spp.; Zygosaccharomyces spp.; molds; yeasts

Description

General description

Yeast Nitrogen Base Without Amino Acids and Ammonium Sulfate is recommended to use for classifying yeasts based on their carbon and nitrogen requirements. The medium contains all essential nutrients required for the growth of yeasts except amino acids, histidine, methionine and tryptophan, and also ammonium sulphate. The addition of nitrogen and carbon sources is required as described in the Manual of Clinical Microbiology

Application

Yeast Nitrogen Base Without Amino Acids and Ammonium Sulfate is used in classifying yeasts based on carbon and nitrogen requirements. It contains all essential nutrients required for the growth of yeasts except amino acids, nitrogen and carbohydrate. The addition of nitrogen and carbon sources is required.

Preparation Note

• Prepare a 10× stock solution by suspending 1.7 g of yeast nitrogen base without amino acids and ammonium sulfate in 100 ml of cold distilled water. Include desired nitrogen and carbon sources.
• Warm if necessary to solubilize and sterilize by filtration.
• Store 10× stock solution at 2-8°C. To use, dilute 1:10 with sterile distilled water under aspetic conditions.

Ingredients
Biotin, 2 μg/L
Calcium pantothenate, 400 μg/L
Folic acid, 2 μg/L
Niacin, 400 μg/L
p-Aminobenzoic acid, 200 μg/L
Pyridoxine HCl, 400 μg/L
Riboflavin, 200 μg/L
Thiamine HCl, 400 μg/L
Inositol, 2 mg/L
Boric acid, 500 μg/L
Copper sulfate, 40 μg/L
Potassium iodide, 100 μg/L
Ferric chloride, 200 μg/L
Manganese sulfate, 400 μg/L
Sodium molybdate, 200 μg/L
Zinc sulfate, 400 μg/L
Potassium phosphate monobasic, 1 g/L
Magnesium sulfate, 0.5 g/L
Sodium chloride, 0.1 g/L
Calcium chloride, 0.1 g/L

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Kit Components Only

• KH₂PO₄ 1 g/L
• MgSO₄ .5 g/L
• NaCl .1 g/L
• CaCl₂ .1 g/L
• Inositol 2 mg/L

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves