V900891

Collagenase from Clostridium histolyticum

Type I, for general use, Vetec^™, reagent grade, powder, ≥125 CDU/mg solid

• Synonym(s): Clostridiopeptidase A
• CAS Number: 9001-12-1
• EC Number: 3.4.24.3 (BRENDA, IUBMB)
• EC Number: 232-582-9
• MDL number: MFCD00130830
• UNSPSC Code: 12352204

Properties

• grade: reagent grade
• product line: Vetec^™
• form: powder
• specific activity: ≥125 CDU/mg solid
• storage temp.: −20°C

Description

Application

Collagenase from Clostridium histolyticum may be used for the isolation of cells from many types of animal tissue. VETEC Collagenase Type I has the original balance of collagenase, caseinase, clostripain, and tryptic activities, and is suggested for epithelial, liver, lung and adrenal primary cell isolations.

Biochem/physiol Actions

Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.

Quality

Also contains clostripain, nonspecific neutral protease, and tryptic activities.

Unit Definition

One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.

Legal Information

Vetec is a trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Danger
• Hazard Statements: H315,H319,H334,H335
• Precautionary Statements: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable