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SRP5280

Sigma-Aldrich

PI3K (p110-α/p85-α), active, His tagged from mouse and human

PRECISIO®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):

p110-alpha, p85-alpha

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About This Item

CAS Number:
UNSPSC Code:
12352200
NACRES:
NA.32
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biological source

human
mouse

recombinant

expressed in baculovirus infected Sf9 cells

product line

PRECISIO®

Assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

64-97 nmol/min·mg

mol wt

~111 kDa (p110α)
~86 kDa (p85α)

NCBI accession no.

application(s)

cell analysis

shipped in

dry ice

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This Item
SRP5295SRP5319SRP0233
biological source

human, mouse

biological source

human

biological source

-

biological source

human

Gene Information

mouse ... Pik3ca(18706)

Gene Information

human ... PIK3CB(5291)

Gene Information

human ... PIK3CA(5290)

Gene Information

human ... PIK3CA(5290)

specific activity

64-97 nmol/min·mg

specific activity

22.4-33.6 nmol/min·mg

specific activity

11.2-16.8 nmol/min·mg

specific activity

≥13 pmol/min-μg

assay

≥70% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

assay

≥80% (SDS-PAGE)

application(s)

cell analysis

application(s)

cell analysis

application(s)

-

application(s)

life science and biopharma

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected insect cells

General description

The PI3K comprises of a 110 kDa catalytic subunit and a 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. The p110 alpha catalytic subunit (PIK3CA) is frequently mutated or amplified in a variety of cancers including ovarian and colon. PIK3CA gene copy number is increased in over 30% of ovarian cancers and this leads to increased PI3-kinase activity. Furthermore, the activity of p110 alpha is essential for vascular development and inactivation of p110 alpha leads to severe defects in angiogenic sprouting and vascular remodeling.

Physical form

Supplied in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, 25% glycerol.

Preparation Note

After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazardExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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    ELOVL4-mediated production of very long-chain ceramides stabilizes tight junctions and prevents diabetes-induced retinal vascular permeability
    Kady NM, et al.
    Diabetes, 67(4), 769-781 (2018)
    Ignazio Restivo et al.
    International journal of molecular sciences, 24(2) (2023-01-22)
    Cell death program of red blood cells (RBCs), called eryptosis, is characterized by activation of caspases and scrambling of membrane phospholipids with externalization of phosphatidylserine (PS). Excessive eryptosis confers a procoagulant phenotype and is implicated in impairment of microcirculation and
    Insulin resistance, ceramide accumulation, and endoplasmic reticulum stress in human chronic alcohol-related liver disease
    Longato L, et al.
    Oxidative Medicine and Cellular Longevity, 2012 (2012)
    Role of sphingomyelinase in mitochondrial ceramide accumulation during reperfusion
    Ramirez-Camacho I, et al.
    Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1862(10), 1955-1963 (2016)
    The sphingolipid salvage pathway in ceramide metabolism and signaling
    Kitatani K, et al.
    Cellular Signalling, 20(6), 1010-1018 (2008)

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