추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
Mab-c48, monoclonal
분자량
calculated mol wt 19.77 kDa
observed mol wt ~40 kDa
정제법
using protein G
종 반응성
human
포장
antibody small pack of 100 μg
기술
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
동형
IgG1κ
에피토프 서열
Extracellular domain
단백질 ID 수납 번호
UniProt 수납 번호
저장 온도
2-8°C
타겟 번역 후 변형
unmodified
유전자 정보
human ... BST2(137964)
일반 설명
Bone marrow stromal antigen 2 (UniProt: Q10589; also known as BST-2, Tetherin, HM1.24 antigen, CD317) is encoded by the BST2 gene (Gene ID: 684) in human. BST-2 is a single-pass type II disulfide-linked homodimeric membrane protein that is synthesized with a propeptide (aa 162-180), which is subsequently cleaved off to produce the mature protein that has a cytoplasmic domain (aa 1-20), a transmembrane domain (aa 21-48), and an extracellular domain (aa 49-161). It is predominantly expressed in liver, lung, heart, placenta, and in T-cells, monocytes, NK cells, and dendritic cells. Over-expression of BST-2 is observed in multiple myeloma cells. Its expression of BST-2 is regulated by both extrinsic and intrinsic stimuli, including interferons. It is reported to shuttle between the cell membrane, where it is present predominantly in membrane/lipid rafts, and the trans-Golgi network. BST-2 serves as an IFN-induced antiviral host restriction factor that efficiently blocks the release of diverse mammalian enveloped viruses by directly tethering nascent virions to the membranes of infected cells. It acts as a direct physical tether, holding virions to the cell membrane and linking virions to each other. The tethered virions can be either internalized by endocytosis and subsequently degraded or they can remain on the cell surface. In either case, their spread as cell-free virions is restricted. Within its extracellular region, it has a coiled coil domain (aa 68-152) that forms an extended 17 nm long semi-flexible rod-like structure, which is essential for virion retention at the cell surface and prevent virus spreading. BST-2 is reported to mediate various facets of cancer progression and metastasis and has been linked to poor survival in invasive breast cancer patients. Its expression positively correlates with disease severity. (Ref.: Mlimi, H., et al. (2022). Retrovirology. 19; Article 3; Mahauad-Fernandez, WD., et al. (2018). Sci. Rep. 8; Article 17608; Ohtomo, T., et al. (1999). Biochem. Biophys. Res. Commun. 258(3); 583-591).
특이성
Clone Mab-c48 is a mouse monoclonal antibody that detects Bone marrow stromal antigen 2 (BST-2). It targets an epitope within the extracellular domain.
면역원
786-O and Caki-1 cells, alternative immunizations
애플리케이션
Quality Control Testing
Evaluated by Flow Cytometry in 786-O cells.
Flow Cytometry Analysis: 1 µg of this antibody detected BST-2 in one million 786-O cells.
Tested Applications
Western Blotting Analysis: 1 µg/mL from a representative lot detected recombinant Human BST-2. (Data courtesy of an independent research laboratory).
Immunoprecipitation Analysis: 1 µg/mL from a representative lot immunoprecipitated BST-2 in 786-O cell lysates. (Data courtesy of an independent research laboratory).
Immunohistochemistry (Paraffin) Analysis: 8 µg/mL from a representative lot detected BST-2 in chromophobe renal cell carcinoma (RCC) tissues. (Data courtesy of an independent research laboratory).
Flow Cytometry Analysis: 1 µg/mL from a representative lot detected BST-2 in 786-O cells (Data courtesy of an independent research laboratory).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Flow Cytometry in 786-O cells.
Flow Cytometry Analysis: 1 µg of this antibody detected BST-2 in one million 786-O cells.
Tested Applications
Western Blotting Analysis: 1 µg/mL from a representative lot detected recombinant Human BST-2. (Data courtesy of an independent research laboratory).
Immunoprecipitation Analysis: 1 µg/mL from a representative lot immunoprecipitated BST-2 in 786-O cell lysates. (Data courtesy of an independent research laboratory).
Immunohistochemistry (Paraffin) Analysis: 8 µg/mL from a representative lot detected BST-2 in chromophobe renal cell carcinoma (RCC) tissues. (Data courtesy of an independent research laboratory).
Flow Cytometry Analysis: 1 µg/mL from a representative lot detected BST-2 in 786-O cells (Data courtesy of an independent research laboratory).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
물리적 형태
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
재구성
1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
저장 및 안정성
Recommended storage: +2°C to +8°C.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
적합한 제품을 찾을 수 없으신가요?
당사의 제품 선택기 도구.을(를) 시도해 보세요.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.