10105031001

β-Galactosidase

from E. coli overproducer

• 동의어(들): β-galactosidase
• EC 번호: 3.2.1.23 (BRENDA, IUBMB)
• UNSPSC 코드: 12352204

속성

• 생물학적 소스: Escherichia coli
• Quality Level: 100
• 양식: suspension
• 분자량: 540 kDa
• 포장: pkg of 1,500 U
• 제조업체/상표: Roche
• 농도: 5.00 mg/mL
• 기술: activity assay: suitable
• 최적 pH: 7
• 적합성: suitable for UV spectrophotometry and general use
• 응용 분야: life science and biopharma
• 배송 상태: wet ice
• 저장 온도: 2-8°C

설명

일반 설명

β-Galactosidase or β-D-Galactoside galactohydrolase is basically a tetramer made up of identical four polypeptide chains, each constituting 1023 amino acids. It is very specific for D-galactose and requires K⁺ or Na⁺ and Mg²⁺ to be fully active. β-Galactosidase enzyme with an oxygen glycosidic bond catalyzes reactions with β-d-galactopyranosides.

At 37 °C with 2-nitrophenyl-β-D-galactoside as the substrate, approximately 30 U/mg at 25 °C with lactose as the substrate; standardized with BSA.

특이성

Cleaves terminal galactose residues that are β1,4-linked to a monosaccharide, oligosaccharide, or glycopeptide.

애플리케이션

β-galactosidase has been used in enzyme-linked immunosorbent assay (ELISA). Use β-Galactosidase to produce a calibration curve in enzymatic assays.

품질

Contaminants: <0.01% GIDH, GPT, LDH, MDH, and oxaloacetate decarboxylase, each

물리적 형태

Suspension, in 3.2 M ammonium sulfate solution, pH approximately 6, crystalline

제조 메모

The ammonium sulfate preparation is stable at 2 to 8 °C until the expiration date printed on the label. Use directly for most applications, e.g., quantitation of lactose.

In the absence of ammonium sulfate, solutions of β-galactosidase should be stabilized with Mg²⁺ (89 mM) and a thiol reagent (1mM β-mercaptoethanol, 1 mM dithiothreitol) [Beutler, 1984]. The thiol slows the formation of enzyme dimers resulting from intramolecular disulfide bridges.

Activator: K (50 mM) is required for activation (lactose hydrolysis).
Na (50 mM) is also an activator, particularly for hydrolysis of 2-nitrophenyl-β-D-galactopyranoside.

기타 정보

For life science research only. Not for use in diagnostic procedures.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): No data available
• Flash Point (°C): No data available