G4155

β-Galactosidase from Escherichia coli

aqueous glycerol suspension, ≥500 units/mg protein (biuret)

• 동의어(들): β-D-Galactoside galactohydrolase, Lactase
• CAS Number: 9031-11-2
• EC 번호: 3.2.1.23 (BRENDA, IUBMB)
• MDL number: MFCD00130623
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 양식: aqueous glycerol suspension
• Quality Level: 200
• 특이 활성도: ≥500 units/mg protein (biuret)
• 분자량: 465 kDa
• 저장 온도: −20°C

설명

애플리케이션

β-Galactosidase is used in the enzymatic assays in the synthesis of imidazolo-pyrrolidinoses.

생화학적/생리학적 작용

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

물리적 특성

Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)

단위 정의

One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.

물리적 형태

Suspension in 50% glycerol, 5 mM Tris buffer salts, 5 mM magnesium chloride, 0.5 mM DTT, 0.5 mM mercaptoethanol. pH 7.4

기타 정보

Loss of enzymatic activity can occur if this enzyme is frozen. This enzyme is provided in a 50% glycerol solution and will not freeze if stored at -20° Celsius. It is not recommended that this enzyme preparation be stored in an ultracold freezer (-60 to -100° Celsius).

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable