10843555001

Hygromycin B

from Streptomyces hygroscopicus

• 동의어(들): Hygromycin B from Streptomyces hygroscopicus
• 실험식(Hill 표기법): C₂₀H₃₇N₃O₁₃
• CAS Number: 31282-04-9
• Molecular Weight: 527.52
• MDL number: MFCD06795479
• UNSPSC 코드: 12352200
• PubChem Substance ID: 329749191

속성

• 생물학적 소스: Streptomyces hygroscopicus
• 무균: non-sterile; 0.2 μm filtered
• 분석: 80% (HPLC)
• 양식: buffered aqueous solution
• 구성: Hygromycin B, >80% HPLC
• 포장: pkg of 20 mL (1 g)
• 제조업체/상표: Roche
• 농도: 50 mg/mL
• 기술: transfection: suitable
• SMILES string: CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3O[C@]4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O
• InChI: 1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1
• InChI key: GRRNUXAQVGOGFE-XKIAHZFYSA-N

설명

일반 설명

• Formula: C₂₀H₃₇N₃O₁₃
• LD50: 6 mg/kg in mouse (i.v.);13 mg/kg in guinea pig (i.p.); 63 mg/kg in rat (i.p.)
• Molecular weight: Mr = 527.5
• Working concentration: 50 - 1,000 μg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 μg/ml. However, the optimal concentration must be experimentally determined, since it may vary depending on the type of cell used.

애플리케이션

Hygromycin B is an aminoglycosidic antibiotic that inhibits protein synthesis in prokaryotes and eukaryotes. It is used to select and maintain the phenotype of eukaryotic cells that are stably transfected with the E. coli hygromycin-resistance gene (hyg or hph).

생화학적/생리학적 작용

Mode of Action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation.

Antimicrobial Spectrum: Hygromycin B acts against bacteria, fungi and higher eukaryotic cells.

품질

Purity: >80% (HPLC)

단위 정의

Unit Assay: Unit/mg
We do not have this information.

물리적 형태

Solution, 50 mg/ml in PBS (phosphate-buffered saline), filtered through 0.2 μm pore size membrane

제조 메모

Working concentration: Recommended concentration for the selection of resistant cells is 50 to 1000 μg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 μg/ml. However, the optimal concentration must be determined experimentally, and may vary with the type of cell used.

저장 및 안정성

Store at 2 to 8 °C. (solution)

기타 정보

For life science research only. Not for use in diagnostic procedures.

Solution, 50 mg/ml, in PBS (phosphate-buffered saline), filtered through 0.2 μm pore size membrane.

안전성 정보

• 픽토그램: GHS05,GHS06,GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H301,H312,H317,H318,H330,H334
• 예방조치 성명서: P260 - P280 - P301 + P310 + P330 - P304 + P340 + P310 - P305 + P351 + P338 + P310 - P342 + P311 - P403 + P233
• Hazard Classifications: Acute Tox. 1 Inhalation - Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Eye Dam. 1 - Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• WGK: WGK 1
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash