11011375001

Nutridoma^™-SP

suitable for hybridoma, solution, pkg of 100 mL (100x)

• 동의어(들): hybridoma, nutridoma
• UNSPSC 코드: 12352207

속성

• 무균: sterile; 0.2 μm filtered
• Quality Level: 100
• 양식: solution
• 포장: pkg of 100 mL (100x)
• 제조업체/상표: Roche
• 농도: 100 ×
• 기술: cell culture | hybridoma: suitable
• 불순물: mycoplasma, tested; ≤100 EU/mL Endotoxin (LAL test)
• solubility: water: miscible
• 저장 온도: 20-25°C

설명

일반 설명

Nutridoma is a family of chemically defined supplements that can completely replace serum in cell culture media. Nutridoma-SP is used for the cultivation of established myeloma and hybridoma cells. It is used for the serum-free cultivation of murine myelomas and hybridomas that have an intact cholesterol biosynthesis pathway, such as those derived from the SP2/0, and some of the P3x63Ag8.653 cell lines and lymphoblastoid cell lines, as well as for primary lymphoid cell cultures. It is also used for the serum-free culture of a variety of other cell types, including neural explants.

애플리케이션

Nutridoma-SP has been used in the culture media for canine trophoblasts, human embryonic stem cells and PLB-985 cells.

단위 정의

Endotoxin (LAL): 10 EU/ml; mycoplasma-tested

물리적 형태

Solution, 100x concentrated, filtered through 0.2 μm pore size membrane
Biochemically defined serum-free medium supplement composed of albumin, insulin, transferrin, and other defined organic and inorganic compounds. Nutridoma contains no other growth factors, mitogens, hormones, or sterols.
The solution contains the pH indicator phenol red and appears clear.

제조 메모

Working concentration: Nutridoma-SP (100x) is diluted 1:100 (v/v) with sterile basal medium. Use high glucose DMEM/RPMI 1640 (1:1) (R/D medium) for Nutridoma-SP. The final medium should also contain L-glutamine and sodium bicarbonate. The protein concentration is less than 50 μg/ml for 1% working concentration.
Storage conditions (working solution): 2 to 8 °C
Nutridoma working solutions, e.g., media containing 1% Nutridoma (1%) are stable for 4 weeks when stored at 2 to 8 °C in the dark. Do not freeze.
Note: Do not store in plastic as adsorption of ingredients to the plastic surface may occur.

기타 정보

For life science research only. Not for use in diagnostic procedures.

법적 정보

Nutridoma is a trademark of Roche

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point (°F): No data available
• Flash Point (°C): No data available