11363743001

Nutridoma^™-CS

suitable for hybridoma, solution, pkg of 10 mL (50x)

• 동의어(들): serum replacements
• UNSPSC 코드: 12352207

속성

• 무균: sterile; 0.2 μm filtered
• Quality Level: 100
• 양식: solution
• 포장: pkg of 10 mL (50x)
• 제조업체/상표: Roche
• 농도: 50 ×
• 기술: cell culture | hybridoma: suitable
• 불순물: mycoplasma, tested; 100 EU/mL endotoxin (LAL test)
• solubility: water: miscible
• 저장 온도: −20°C

설명

일반 설명

Nutridoma-CS is a serum replacement solution which helps in cell proliferation when added to a suspension. Its major component is β-D xylopyranose which has ring shaped sugar allowing the cells to grow in a suspension even without serum.

애플리케이션

Contents
Solution, 50× concentrated, Biochemically defined, filtered through 0.2μm pore size membrane, serum-free medium supplement composed of albumin, insulin, transferrin, several cytokines, a cholesterol source, and other defined organic and inorganic compounds. Nutridoma-CS contains human proteins.

Biochemically defined supplement for the replacement of fetal bovine serum (FBS) in cultures of hybridoma cells. Nutridoma-CS supports the growth of SP2/0-, P3x63Ag8.653-, and NS-1-derived hybridomas. It is specifically formulated to optimize the growth of freshly fused hybridomas during selection and cloning procedures in serum-free cell culture without feeder cells.

Nutridoma^™-CS has been used as a supplement in neurobasal medium for culturing human embryonic stem cell (hESC) lines.

단위 정의

Endotoxin (LAL): 100 EU/ml, mycoplasma tested

물리적 형태

Solution (50x concentrated, pH 7.4); sterile. The protein concentration is less than 1 mg/ml for 2% (1x) working concentration.

제조 메모

Working concentration: Nutridoma-CS (50x) is diluted 1:50 (v/v) in basal medium. It is strongly suggested to use RPMI 1640. The final protein concentration is less than 1 mg/ml. The final medium should also contain L-glutamine and β-mercaptoethanol.

기타 정보

For life science research only. Not for use in diagnostic procedures.

법적 정보

Nutridoma is a trademark of Roche

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash