11204530001

ABTS^™ Buffer

solution, pkg of 125 mL

• 동의어(들): buffer
• UNSPSC 코드: 12352204

속성

• 양식: solution
• Quality Level: 100
• 포장: pkg of 125 mL
• 제조업체/상표: Roche
• 배송 상태: wet ice
• 저장 온도: 2-8°C

설명

일반 설명

2,2′-azino-di-(3-ethylbenzthiazoline sulfonic acid) or ABTS acts as a substrate for HRP (horseradish peroxidase) conjugate during enzyme-linked immunosorbent assay (ELISA). It is the most sensitive, and stable substrate when compared to three other substrates namely, 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT). It also produces the best visual results, where it gives a bluish-green color. ELISA using ABTS is a highly sensitive, specific and reproducible technique.

애플리케이션

ABTS Buffer is a solvent for ABTS. ABTS solution is the ideal substrate solution for enzyme immunoassays with horseradish peroxidase (HRP) as a marker enzyme. Because of the high sensitivity of the reaction, autoreaders will often display "over" after several minutes.

제조 메모

Working concentration: Flow cytometry: 0.5 μg/100 μl (106 cells); Immunohistocytochemistry: 50 μg/ml
Working concentration of conjugate depends on application and substrate. Concentrations should be taken as a guideline.
Working solution: Dissolve one ABTS tablet (5 mg) in 5 ml of ABTS buffer. This solution has a light-green color. In microtiter plates, the solution is colorless A_{405 nm/1 cm} should be <0.16. Work as clean as possible. Heavy metal ions or traces of peroxidase can produce a dark green ABTS solution. Such solutions are not suitable for the assay.

기타 정보

For life science research only. Not for use in diagnostic procedures.

법적 정보

ABTS is a trademark of Roche

안전성 정보

• WGK: nwg
• Flash Point (°F): No data available
• Flash Point (°C): No data available