11333089001

Anti-Digoxigenin

from sheep

• 동의어(들): anti-digoxigenin, digoxigenin
• UNSPSC 코드: 12352203

속성

• 생물학적 소스: sheep
• Quality Level: 100
• 결합: unconjugated
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 양식: lyophilized
• 포장: pkg of 200 μg
• 제조업체/상표: Roche
• 동형: IgG
• 저장 온도: 2-8°C

설명

일반 설명

Digoxigenin is a hapten which is used in labeling of nucleic acids and in detection systems.

Probes labeled with digoxigenin has greater sensitivity equivalent to that of radioactive probes. It allows faster detection, is less hazardous and has an increased shelf life.

특이성

The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.

애플리케이션

Anti-Digoxigenin has been used in DNA tethering. It has been used to attach DNA molecule to the glass surface of the flow cell.

Use Anti-Digoxigenin antibody for the detection of digoxigenin-labeled compounds using:

• ELISA
• Immunohistocytochemistry
• In situ hybridization
• Western blot

생화학적/생리학적 작용

In the presence of Na⁺, Mg²⁺ and ATP, digoxigenin inhibits sodium pumps.

제조 메모

Working concentration: Working concentration of antibody depends on application and substrate. The following concentrations should be taken as a guideline:

• ELISA: for coating: 2 to 4 μg/ml
• Immunohistocytochemistry: 0.5 to 2 μg/ml
• In situ hybridization: 0.2 to 0.4 μg/ml
• Western blot: 0.5 to 2 μg/ml

Working solution: For coating applications: PBS (phosphate buffered saline), pH 7.4

After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption.

재구성

Add 1 ml PBS to a final concentration of 200 μg/ml.

분석 메모

No cross reactivity with other steroids, such as human estrogens e.g., estradiol or androgens e.g., testosterone.

기타 정보

For life science research only. Not for use in diagnostic procedures.

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H317
• 예방조치 성명서: P261 - P272 - P280 - P333 + P313 - P362 + P364 - P501
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash