11418165001

Biotin Protein Labeling Kit

sufficient for 5 labeling reactions, kit of 1 (5 components), suitable for immunoblotting, suitable for ELISA

• 동의어(들): Biotin, protein labeling
• UNSPSC 코드: 12352200

속성

• 사용: sufficient for 5 labeling reactions
• Quality Level: 100
• 포장: kit of 1 (5 components)
• 제조업체/상표: Roche
• 기술: ELISA: suitable; immunoblotting: suitable
• 저장 온도: 2-8°C

설명

일반 설명

For labeling of proteins with biotin.
Free amino acids of the protein to be labeled react with D-biotinoyl-ε-aminocaproic acid-N-hydroxy-succinimide ester (biotin-7-NHS) by forming a stable amino bond. Non reacted biotin-7-NHS is separated on a Sephadex G-25 column.

애플리케이션

Biotin protein labeling kit has been used to produce biotinylated denatured bovine serum albumin (d-BSA) for coating cadmium telluride (CdTe) quantum dot (QD) conjugates.
The kit is suitable for the labeling of proteins with biotin for immunochemistry, immunoblotting, and ELISA.

포장

1 kit containing 5 components.

품질

Function tested.

제조 메모

Working solution: Preparation of the solutions

• Blocking solution: Dissolve the contents of the bottle blocking reagent (green screw cap) in 300 ml double-distilled water.
• PBS solution: Dissolve the contents of the bottle PBS (blue screw cap) in 1 I double-distilled water.
• Biotin-7-NHS solution: The solution should be freshly prepared before labeling. Add to the contents of the reaction vial which includes biotin-7-NHS 250 μI DMSO and vortex the solution several times. The resulting concentration of the biotin-7-NHS solution is 20 mg/ml. For the labeling mix, the volume of the biotin-7-NHS solution should not exceed 5% of the total reaction volume (e.g., add maximal 50 μl biotin-7-NHS solution to 1 ml protein solution).

기타 정보

For life science research only. Not for use in diagnostic procedures.

키트 구성품 전용

• Blocking Reagent (green screw cap)
• Phosphate Buffered Saline (PBS) (blue screw cap)
• D-Biotinoyl-ε-aminocaproic acid-N-hydroxysuccinimide ester (Biotin-7-NHS)
• Dimethylsulfoxide (DMSO) (red screw cap)
• Sephadex G-25 Columns

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1