10771

Histopaque^®-1077

sterile-filtered, density: 1.077

• UNSPSC 코드: 12352207
• NACRES: NA.75

속성

• 무균: sterile-filtered
• 양식: liquid
• 유통기한: Expiry date on the label.
• 기술: cell culture | mammalian: suitable
• 불순물: endotoxin, tested
• density: 1.077
• 응용 분야: hematology; histology
• 저장 온도: 2-8°C

설명

일반 설명

Histopaque-1077 is a sterile, endotoxin tested, ready-to-use medium. The Histopaque composition includes polysucrose and sodium diatrizoate, adjusted to a relative density of 1.077g/mL. This ready-to-use medium facilitates rapid recovery of viable lymphocytes and other mononuclear cells from small volumes of whole blood or bone marrow.

애플리케이션

Histopaque-1077 has been used for:

• human lymphocyte antigen (HLA) typing
• initial isolation step prior to the enumeration of T, B, and ‘null’ lymphocytes.
• preparation of pure lymphocyte suspensions for cell culture and cytotoxicity assays.
• isolation and purification of peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs)
• isolation of human lymphocytes
• isolation of breast cancer circulating tumor cells (CTCs) from the whole blood
• Histopaque-1077 is used in conjunction with Histopaque-1119 as it facilitates the rapid recovery of viable mononuclear cells and granulocytes from small volumes of whole blood

특징 및 장점

Histopaque^®-1077 offers numerous advantages, such as:

• Optimal recovery of viable cells
• Permits selective separation of blood cell types
• Prevents cell distortion
• Preserves cell viability
• Minimal extraneous cell interference
• Reproducible performance from batch to batch
• Sterile-filtered and endotoxin-tested
• Stable at 2-8°C for at least 2 years when protected from light

원리

Anticoagulated blood is layered onto Histopaque-1077. During centrifugation, erythrocytes are aggregated by polysucrose and rapidly sediment. Granulocytes become slightly hypertonic, which increases their sedimentation rate, resulting in pelleting at the bottom of the centrifuge tube. Lymphocytes and other mononuclear cells remain at the plasma/ Histopaque-1077 interface.

법적 정보

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H317,H334
• 예방조치 성명서: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3