11191

Histopaque^®-1119

sterile-filtered, density: 1.119

• UNSPSC 코드: 12352207
• NACRES: NA.75

속성

• 무균: sterile-filtered
• 양식: liquid
• 유통기한: Expiry date on the label.
• 기술: cell culture | mammalian: suitable
• 불순물: endotoxin, tested
• density: 1.119
• 응용 분야: hematology; histology
• 저장 온도: 2-8°C

설명

일반 설명

Histopaque^®-1119 is a sterile, endotoxin tested, ready-to-use medium. This solution consists of polysucrose and sodium diatrizoate, adjusted to a relative density of 1.119 g/mL and pH 8.8–9.0.

애플리케이션

Histopaque^®-1119 is suitable for use in conjunction with Histopaque-1077 as it facilitates the rapid recovery of viable mononuclear cells and granulocytes from small volumes of whole blood or bone marrow

특징 및 장점

Histopaque^®-1119 offers numerous advantages, such as:

• Optimal recovery of viable cells
• Permits selective separation of blood cell types
• Prevents cell distortion
• Preserves cell viability
• Minimal extraneous cell interference
• Reproducible performance from batch to batch
• Sterile-filtered and endotoxin-tested
• Stable at 2-8°C for at least 2 years when protected from light

원리

The process involves the formation of a double gradient on layering an equal volume of Histopaque-1077 over Histopaque-1119. Anticoagulated whole blood is carefully layered onto the upper Histopaque-1077 medium. Upon centrifugation, the erythrocytes are aggregated by polysucrose and thus rapidly sediment. Granulocytes are found at the lower Histopaque-1077/1119 interface; whereas, lymphocytes and other mononuclear cells are found at the upper plasma/Histopaque-1077 interface. Most extraneous platelets found in the upper interface are removed by low-speed centrifugation during washing steps. Erythrocyte contamination is negligible.

법적 정보

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• 픽토그램: GHS05
• 신호어: Warning
• 유해 및 위험 성명서: H290,H315,H319
• 예방조치 성명서: P234 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313
• Hazard Classifications: Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
• Storage Class Code: 8A - Combustible corrosive hazardous materials
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable