4381663

iTRAQ^® Reagent - 8PLEX Multiplex Kit

• 동의어(들): iTRAQ 8-Plex
• UNSPSC 코드: 12161503
• NACRES: NA.24

속성

• Quality Level: 100
• 저장 온도: −20°C

설명

일반 설명

Contains iTRAQ^® Reagents 113, 114, 115, 116, 117, 118, 119 and 121 and sufficient material for five 8-plex assays. Multiplex Buffer kit (Cat. No. 4381664) to be ordered separately.

Cat. No. 4381663 contains the equivalent reagents in the Sciex iChemistry^® product number 4390812.

The isobaric tags for relative and absolute quantitation (iTRAQ^®) reagents are the first set of multiplexed, amine-specific, stable-isotope reagents that can label all peptides in up to eight different biological samples enabling simultaneous identification and quantitation, relative and absolute, while retaining important post-translational modification (PTM) information. The iTRAQ Reagents - 8plex kits are available in more economical bulk packs.

애플리케이션

iTRAQ^® Reagent - 8PLEX Multiplex Kit has been used:

• to derivatize and label the tryptic digests of the iodoacetamide probe (IPM)-labeled HeLa proteome
• in isobaric tags for relative and absolute quantitation (iTRAQ) labeling of peptides
• as isobaric labels for the comparative quantification of proteins between the digested samples in proteomic analysis

특징 및 장점

• Simultaneously identify and quantify proteins from multiple samples
• Expands protein and proteome coverage by labeling all peptides, including those with post-translational modifications (PTMs)
• Increases confidence in identification and quantitation from MS/MS spectra by tagging multiple peptides per protein
• Increases throughput and confidence in results for protein biomarker discovery studies
• Offers a simple workflow without sample fractionation for reduced-complexity samples, such as affinity pull-downs
• Provides the flexibility to multiplex up to eight different biological samples simultaneously in a single experiment
• Fully supported by ProteinPilot Software on all SCIEX proteomics LC/MS/MS platforms

분석 메모

To view the Protocol for the iTRAQ Reagents 8-plex Kit, please visit this Protocol link.

To view the Chemistry Quick Reference Card for the iTRAQ Reagents, please visit this Chemistry Quick Reference Card link.

법적 정보

iChemistry is a registered trademark of AB Sciex Pte. Ltd.

iTraq is a registered trademark of AB Sciex Pte. Ltd.

안전성 정보

• 픽토그램: GHS02,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H225,H302 + H312 + H332,H319
• 예방조치 성명서: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2
• Storage Class Code: 3 - Flammable liquids
• Flash Point (°F): 35.6 °F - closed cup
• Flash Point (°C): 2.0 °C - closed cup