59767

Chlorophenol Red-β-D-galactopyranoside

β-galactosidase, ≥90% (HPLC), powder or crystals

• 동의어(들): CPRG, Chlorophenol Red-β-D-galactoside
• 실험식(Hill 표기법): C₂₅H₂₂Cl₂O₁₀S
• CAS Number: 99792-79-7
• Molecular Weight: 585.41
• MDL number: MFCD05863726
• UNSPSC 코드: 12352204
• PubChem Substance ID: 57651696
• NACRES: NA.83

속성

• Product Name: Chlorophenol Red-β-D-galactopyranoside, ≥90% (HPLC)
• 분석: ≥90% (HPLC)
• 양식: powder or crystals
• solubility: water: 20 mg/mL, clear, orange to very deep red
• 저장 온도: −20°C
• SMILES string: OC[C@H]1O[C@@H](Oc2ccc(cc2Cl)C(=C3/C=CC(=O)C(Cl)=C3)\c4ccccc4S(O)(=O)=O)[C@H](O)[C@@H](O)[C@H]1O
• InChI: 1S/C25H22Cl2O10S/c26-15-9-12(5-7-17(15)29)21(14-3-1-2-4-20(14)38(33,34)35)13-6-8-18(16(27)10-13)36-25-24(32)23(31)22(30)19(11-28)37-25/h1-10,19,22-25,28,30-32H,11H2,(H,33,34,35)/b21-12-/t19-,22+,23+,24-,25-/m1/s1
• InChI key: YOUWVNCQJOMMEU-AETRGLENSA-N

설명

일반 설명

Chlorophenol red-β-D-galactopyranoside (CPRG) is a long-wavelength yellow dye commonly used for colorimetric assays. It serves as a substrate for β-galactosidase (β-gal) which hydrolyzes CPRG to form chlorophenol red (purple colored). This product is quantified by measuring its absorbance at 570 nm. CPRG is used to identify fecal coliform bacteria. It is about 10 times more sensitive than other chromogenic agents such as o-nitrophenyl-β-galactoside (ONPG). CPRG also has a high rapid turnover.

애플리케이션

Chlorophenol Red-β-D-galactopyranoside has been used as a substrate for β-galactosidase (β-gal):

• in paper-based cell-free protein expression system for target RNA detection
• in colorimetric detection of multiple clinically relevant mutations using a lacZ output gene
• in colorimetric assay for bacteria quantification

기타 정보

Measures the expression levels of β-galactosidase in transfected cells. The lacZ gene is a popular reporter gene in transfection reactions because its product, β-galactosidase, is a very stable enzyme.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)