61305

Penicillinase from Bacillus cereus

lyophilized, powder, white, ~13 U/mg

• 동의어(들): β-Lactamase I from Bacillus cereus, β-Lactamase II, Cephalosporinase, Penicillin amido-β-lactam hydrolase
• CAS Number: 9073-60-3
• EC 번호: 3.5.2.6 (BRENDA, IUBMB)
• MDL number: MFCD00131819
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: Bacillus sp. (Bacillus cereus)
• 양식: powder
• 품질: lyophilized
• 특이 활성도: ~13 U/mg
• 분자량: M_r ~30000
• 기술: activity assay: suitable
• 색상: white
• 적합성: suitable for component for culture media
• 응용 분야: diagnostic assay manufacturing
• 외래 활성: β-lactamase II ≤2%
• 저장 온도: −20°C

설명

일반 설명

Penicillinase from Bacillus cereus is both an extracellular and cell-bound enzyme. It has a single polypeptide chain and has a molecular weight of 31 kDa. It lacks cysteine in the protein sequence.

애플리케이션

Penicillinase from Bacillus cereus may be used in the preparation of biosensor for amperometric detection of hydrolysis of penicillin.

Used in the production of penicillin.

생화학적/생리학적 작용

Penicillinase specifically catalyzes the hydrolysis of β-lactam ring of penicillin.

포장

Bottomless glass bottle. Contents are inside inserted fused cone.

단위 정의

One unit will hydrolyze 1.0 μmole of indicated substrate per min at pH 7.0 at 25 °C. Sold as benzylpenicillin units. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.

기타 정보

Used to destroy β-lactam antibiotics in body fluids or culture media. For the determination of penicillin; In the coupled enzyme assay for isopenicillin N synthetase; ELISA of estradiol; Penicillinase optodes.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H317,H334
• 예방조치 성명서: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: dust mask type N95 (US), Eyeshields, Faceshields, Gloves