P4524

β-Lactamase from Enterobacter cloacae

Type IV, lyophilized powder, 0.2-0.6 units/mg protein (using benzylpenicillin)

• 동의어(들): β-Lactamase I, β-Lactamase II, Cephalosporinase, Penicillin amido-β-lactam hydrolase, Penicillinase from Enterobacter cloacae
• CAS Number: 9073-60-3
• EC 번호: 3.5.2.6 (BRENDA, IUBMB)
• MDL number: MFCD00131819
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: bacterial (Enterobacter cloacae)
• Quality Level: 200
• 유형: Type IV
• 양식: lyophilized powder
• 특이 활성도: 0.2-0.6 units/mg protein (using benzylpenicillin)
• 분자량: 29 kDa
• 구성: Protein, ~60%
• 기술: enzyme immunoassay: suitable
• 적합성: suitable for enzyme test
• 응용 분야: pharmaceutical
• 저장 온도: 2-8°C

설명

일반 설명

β-Lactamase is a monomeric enzyme of 29 kDa. This product is produced from Enterobacter cloacae and is provided as a lyophilized powder. It acts as a target protein for β-lactam antibiotics. This enzyme is present in Gram-negative bacteria.

애플리케이션

β--lactamase is used to inactivate β-lactam antibiotics by breaking open the β-lactam ring. β--lactamase is used to study antibiotic resistance and resistance suppression. Product P4524 is produced from Enterobacter cloacae and is provided as a lyophilized powder.

β-Lactamase from Enterobacter cloacae has been used in the maternal and fetal quantitative blood cultures to avoid any carryover phenomenon. It has also been used as a component in Mueller Hinton (MH) broth for placental cultures.

생화학적/생리학적 작용

β--lactamase inactivates β-lactam antibiotics by breaking open the β-lactam ring.

단위 정의

One unit will hydrolyze 1.0 μmole of benzylpenicillin per min at pH 7.0 at 25 °C. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.

물리적 형태

Lyophilized powder containing sodium phosphate buffer salts

분석 메모

Protein determined by biuret.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H317,H334
• 예방조치 성명서: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: dust mask type N95 (US), Eyeshields, Faceshields, Gloves