A9594

Monoclonal ANTI-FLAG^® M2-Cy3^™ antibody produced in mouse

clone M2, purified immunoglobulin, buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)

• 동의어(들): Monoclonal ANTI-FLAG^® M2 antibody produced in mouse, Anti-ddddk, Anti-dykddddk
• UNSPSC 코드: 41106514
• NACRES: NA.32

속성

• 생물학적 소스: mouse
• 결합: CY3 conjugate
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: M2, monoclonal
• 양식: buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)
• 종 반응성: all
• 농도: ~1 mg/mL
• 기술: direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone
• 동형: IgG1
• 면역원 서열: DYKDDDDK
• 배송 상태: dry ice
• 저장 온도: −20°C

설명

일반 설명

Monoclonal ANTI-FLAG M2-Cy3 (mouse IgG) antibody is covalently conjugated to cyanine dye Cy3. The antibody conjugate binds to FLAG fusion proteins, and will recognize the FLAG sequence at the N-terminus, Met-N-terminus, or C-terminus of FLAG fusion proteins.

애플리케이션

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

For simple, one-step detection by immunocytochemistry. Especially useful in detection of FLAG fusion proteins expressed in murine host, where secondary anti-mouse antibodies might cause cross-reactivity.

Learn more product details in our FLAG^® application portal.

물리적 형태

Solution in phosphate buffered saline plus 1% BSA and preservative

제조 메모

Dilute the antibody in Tris buffered saline (TBS): 0.05 M Tris,pH 7.4, with 0.15 M NaCl.

기타 정보

Suggested concentration of 1-10 mg/ml for immunocytochemistry.

법적 정보

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Cy3 is a trademark of Cytiva

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable