F3040

Monoclonal ANTI-FLAG^® M1 antibody

clone M1, purified immunoglobulin, buffered aqueous solution

• 동의어(들): Anti-ddddk, Anti-dykddddk
• MDL number: MFCD00212941
• UNSPSC 코드: 12352203
• NACRES: NA.32

속성

• 생물학적 소스: (Bioreactor)
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: M1, monoclonal
• 양식: buffered aqueous solution
• 정제법: using Protein A
• 종 반응성: all
• 농도: 2-5 mg/mL
• 기술: western blot: 10 μg/mL
• 면역원 서열: DYKDDDDK
• 배송 상태: dry ice
• 저장 온도: −20°C

설명

일반 설명

The ANTI-FLAG M1 IgG2b mooclonal antibody binds to proteins with a FLAG marker at the free N-terminus.

Method of purification - Protein A

면역원

FLAG; peptide sequence DYKDDDDK

애플리케이션

Monoclonal ANTI-FLAG^® M1 antibody is suitable for immunoprecipitation, western blotting, or EIA in yeast, animal and E.coli cells.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.


Chromatin immunoprecipitation (1 paper)


Immunofluorescence (1 paper)

Learn more product details in our FLAG^® applications portal.

생화학적/생리학적 작용

Binds to the FLAG epitope when it is located at the free amino-terminus of a fusion protein. Does not bind to Met-FLAG fusion proteins, so will not recognize unprocessed, cytoplasmically expressed proteins. Binding is Ca²⁺-dependent; the complex dissociates in the absence of calcium ions.

물리적 형태

Solution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v).

제조 메모

Dilute antibody to 10ug/mL in .05 M TBS, pH 7.4, containing 1 mM CaCl2.

기타 정보

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

법적 정보

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable