B2904

Bluo-Gal

reagent for selection of recombinant bacterial clones

• 동의어(들): 5-Bromo-3-indolyl β-D-galactopyranoside, substrate for β-galactosidase, Bluo-Gal
• 실험식(Hill 표기법): C₁₄H₁₆BrNO₆
• CAS Number: 97753-82-7
• Molecular Weight: 374.18
• Beilstein: 1550140
• MDL number: MFCD00063691
• UNSPSC 코드: 12352200
• PubChem Substance ID: 24891417
• NACRES: NA.85

속성

• Grade: Molecular Biology
• Quality Level: 200
• 무균: non-sterile
• 분석: ≥98% (HPLC)
• 양식: powder
• 기술: nucleic acid detection: suitable
• solubility: DMF: soluble
• 적합성: suitable for substrate for β-galactosidase
• 저장 온도: −20°C
• SMILES string: OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O
• InChI: 1S/C14H16BrNO6/c15-6-1-2-8-7(3-6)9(4-16-8)21-14-13(20)12(19)11(18)10(5-17)22-14/h1-4,10-14,16-20H,5H2/t10-,11+,12+,13-,14-/m1/s1
• InChI key: LINMATFDVHBYOS-MBJXGIAVSA-N

설명

일반 설명

Bluo-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Bluo-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

애플리케이션

Bluo-Gal is suitable for use in selection of recombinant bacterial colonies with the lac⁺ phenotype. Bluo-Gal is suitable for use as β-galactosidase substrate in the staining of skin tissues for electron microscopy analysis, Dictyostelium samples and in the staining of vena cava of adult mice.

Chromogenic substrate suitable for identification of lac⁺ bacterial colonies. An alternative to 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal), producing a darker blue color.

원리

When Bluo-Gal is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of Bluo-Gal turn a deeper blue color more intense than X-Gal, allowing for easy differentiation between lac+ and lac- colonies.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)