B3670

Betacellulin human

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• 동의어(들): BTC
• CAS Number: 163150-12-7
• MDL number: MFCD00676521
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• Product Name: Betacellulin human, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture, ≥97% (SDS-PAGE)
• 생물학적 소스: human
• Quality Level: 100
• 재조합: expressed in E. coli
• 분석: ≥97% (SDS-PAGE)
• 양식: lyophilized powder
• 효능: 0.1-0.6 ng/mL ED₅₀
• 분자량: protein 9.5 kDa
• 포장: pkg of 10 μg
• 저장 조건: avoid repeated freeze/thaw cycles
• 기술: cell culture | mammalian: suitable
• 불순물: endotoxin, tested
• UniProt 수납 번호: P35070
• 저장 온도: −20°C
• 유전자 정보: human ... BTC(685)

설명

애플리케이션

This product has been used to study differentiation of pancreatic acinar cell line (AR42J) into insulin-secreting cells and has been linked to a role in the growth and differentiation of islet cells in human pancreas.

생화학적/생리학적 작용

Betacellulin, and the other members of the EGF family of growth factors, binds with high affinity to both EGFR and to erbB-4. Overexpression of Betacellulin with erbB-3 and erbB-4 has been linked to human endometrial adenocarcinoma.

주의사항

This product should be stored at -20°C. After preparation, the product can be stored at 2-8°C for one month, or frozen in aliquots at -20°C or -70°C for more extended storage.

제조 메모

This product was lyophilized from a 0.2 μm filtered solution of PBS containing 5 mg of bovine serum albumin. It should be reconstituted by adding sterile PBS containing 0.1% HAS or BSA to a concentration greater than 1 μg/mL.

분석 메모

The biological activity of this product is measured by an ability to stimulate H-thmidine incorporation in EGF-responsive mouse BALB/c 3T3 fibroblasts. The EC₅₀ is defined as the effective concentration of growth factor that elicits a 50% increase in cell growth in a cell based bioassay.

안전성 정보

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)