추천 제품
Product Name
BCIP®/NBT-Purple Liquid Substrate System for Membranes, ready to use alkaline phosphatase substrate
Quality Level
양식
liquid
저장 온도
2-8°C
애플리케이션
BCIP®/NBT-Purple Liquid Substrate System for Membranes has been used as a substrate for alkaline phosphatase
- for the detection of secondary antibodies by immunoblot
- in ELISPOT assay
- in dot blot analysis
- in western blot to visualize protein bands on nitrocellulose membrane
생화학적/생리학적 작용
5-bromo-4-chloro-3-indolyl phosphate (BCIP)/ nitro blue tetrazolium (NBT) is a chromogenic substrate system for the detection of alkaline phosphatase (AP) enzyme. AP hydrolyses BCIP to form an intermediate leucoindigo compound and is further oxidized by NBT to form 5,5′-dibromo-4,4′-dichloro indigo (BCI). NBT is reduced to an insoluble purple diformazan (DF) and forms NBT-DF. A mixture of NBT-DF and BCI results in the dark purple colored precipitate. AP by hydrolysing many BCIP molecules makes it a highly sensitive method.
주의사항
Stable at least one year at 2-8 °C.
물리적 형태
Ready-to-use.
법적 정보
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Skin Sens. 1
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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