C0278

Chloroperoxidase from Caldariomyces fumago

buffered aqueous suspension, ≥3,000 units/mL

• 동의어(들): Chloride Peroxidase, Chloride:hydrogen-peroxide oxidoreductase
• CAS Number: 9055-20-3
• EC 번호: 1.11.1.10 (BRENDA, IUBMB)
• MDL number: MFCD00130774
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 양식: buffered aqueous suspension
• Quality Level: 200
• 분자량: 42 kDa
• 농도: ≥3,000 units/mL
• 배송 상태: wet ice
• 저장 온도: 2-8°C

설명

일반 설명

Chloroperoxidase is a heme containing glycoprotein that is secreted from fungus. Chloroperoxidase (CPO) is a extracellular heme glycoenzyme containing ferriprotoporphyrin IX as the prosthetic group.

애플리케이션

A useful alternative to lactoperoxidase for ¹³¹I ion labeling studies, for bromination of proteins, and for ³⁶Cl labeling of macromolecules in long-term isolation procedures.

Chloroperoxidase from Caldariomyces fumagois is useful alternative to lactoperoxidase for ¹³¹I ion labeling studies, for bromination of proteins, and for ³⁶Cl labeling of macromolecules in long-term isolation procedures. It has been used to study biocatalytic oxidation in polymersome nanoreactors .

It has been used to study biocatalytic oxidation in polymersome nanoreactors.

생화학적/생리학적 작용

Chloroperoxidase (CPO) is secreted from fungus and exhibits a broad spectrum of chemical reactivities. It is a peroxide-dependent chlorinating enzyme and it also catalyzes peroxidase, catalase and cytochrome P450-type reactions of dehydrogenation, hydrogenperoxide (H₂O₂) decomposition and oxygen insertion, respectively. The enzyme has magnetic and spectroscopic properties similar to that of cyctochrome P-450. CPO from the fungus Caldariomyces fumago has the capacity to chlorinate aromatic hydrocarbons, including polycyclic aromatic hydrocarbons (PAHs). PAHs are considered to be a potential health risk because of their possible carcinogenic and mutagenic activities and are widely dispersed in the environment.

단위 정의

One unit will catalyze the conversion of 1.0 μmole of monochlorodimedon to dichlorodimedon per min at pH 2.75 at 25 °C in the presence of potassium chloride and H₂O₂.

물리적 형태

Crude suspension in 0.1 M sodium phosphate, pH ~4.5

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)