C1057

Butyrylcholinesterase from equine serum

lyophilized powder, ≥900 units/mg protein

• 동의어(들): Acylcholine acyl-hydrolase, Choline esterase, butyryl, Pseudocholinesterase
• CAS Number: 9001-08-5
• EC 번호: 3.1.1.8 (BRENDA, IUBMB)
• EC Number: 232-579-2
• MDL number: MFCD00081455
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 양식: lyophilized powder
• 특이 활성도: ≥900 units/mg protein
• 분자량: tetramer 440 kDa
• 구성: Protein, ≥10%
• solubility: H₂O: soluble 10 mg/mL
• 응용 분야: diagnostic assay manufacturing
• 저장 온도: −20°C

설명

애플리케이션

Selective inhibition of BChE activity can be used in the detection of organophosphates. Its use in the treatment of organophosphate toxicity has been explored. It has been reported that the level of BChE in human blood correlates to the degree of protection against potentially toxic nerve agents. Cholinesterases have also been investigated for their role in Alzheimer′s disease.

생화학적/생리학적 작용

Butyrylcholinesterase (BChE) is a serine hydrolase that is structurally similar to acetylcholinesterase (AChE), but differs in substrate specificities and inhibitor sensitivities.BChE can, unlike AChE, efficiently hydrolyze larger esters of choline such as butyrylcholine and benzoylcholine. The enzyme is a tetrameric glycoprotein with four equal subunits (110 kDa each). The enzyme is activated by Ca²⁺ and Mg²⁺. The activity is constant over the pH range of 6.0-8.0. It is inhibited by betaine, nicotine, organophosphates, and carbamate.

단위 정의

One unit will hydrolyze 1.0 μmole of butyrylcholine to choline and butyrate per min at pH 8.0 at 37 °C. The activity obtained using butyrylcholine as substrate is ~2.5 times that obtained using acetylcholine.

물리적 형태

Highly purified; contains buffer salts

분석 메모

Protein determined by biuret

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)