C4018

Choloylglycine Hydrolase from Clostridium perfringens (C. welchii)

lyophilized powder, ≥100 units/mg protein

• 동의어(들): 3α,7α,12α-Trihydroxy-5β-cholan-24-oylglycine amidohydrolase
• CAS Number: 37289-07-9
• EC 번호: 3.5.1.24 (BRENDA, IUBMB)
• MDL number: MFCD00130789
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 양식: lyophilized powder
• Quality Level: 200
• 특이 활성도: ≥100 units/mg protein
• 구성: Protein, ≥50% biuret
• 배송 상태: wet ice
• 저장 온도: −20°C

설명

애플리케이션

The enzyme from Sigma has been used in the analysis of bile samples in various studies.

생화학적/생리학적 작용

The enzyme is an N-terminal nucleophilic (Ntn) hydrolase that catalyzes the hydrolysis of amide bonds, liberates the glycine/taurine moiety from the steroid core and eventually yields unconjugated bile acids. Agents that oxidize thiol groups (e.g., p-mercuribenzoate, iodoacetamide, Hg²⁺, Cu²⁺, and Cd²⁺) have been shown to strongly inhibit bile salt hydrolase (BSH) activity in Clostridium perfringens.

단위 정의

One unit will hydrolyze 1.0 μmole of glycocholic acid to glycine and cholic acid in 5 min at pH 5.6 at 37 °C.

물리적 형태

Partially purified lyophilized powder containing buffer salts and stabilizer

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)