C7774

Human Collagen Type I

from human placenta, powder, suitable for cell culture

• CAS Number: 9007-34-5
• EC Number: 232-697-4
• MDL number: MFCD00130825
• UNSPSC 코드: 12352202
• NACRES: NA.61

속성

• Product Name: Collagen from human placenta, Bornstein and Traub Type I (Sigma Type VIII), powder
• 생물학적 소스: human placenta
• 양식: powder
• 기술: cell culture | mammalian: suitable
• 불순물: HIV, hepatitis B and hepatitis C, none detected
• UniProt 수납 번호: P02666; P02668; P53420; Q01955; Q14031
• 저장 온도: 2-8°C
• 유전자 정보: bovine ... CSN2(281099), CSN3(281728) ; human ... COL4A3(1285), COL4A4(1286), COL4A6(1288)

설명

애플리케이션

This product is intended to produce thin layer coatings on tissue culture plates to facilitate attachment of anchorage-dependent cells, recommended for use at 6-10 μg/cm². It is NOT intended for production of 3-D gels. Type I collagen is often used in cell culture as an attachment substratum with myoblasts, spinal ganglia, hepatocytes, embryonic lung, heart explants, fibroblasts, endothelial cells, and islet cells have all been cultured successfully on films or gels of type I collagen. Collagen type I may also be used in research of Idiopathic pulmonary fibrosis (IPF), studies on the effect of ER stress IPF on lung fibroblasts. Collagen in acidic solution can produce three dimensional scaffolding with use in bioengineering and cell culture applications.

생화학적/생리학적 작용

Type I collagen is a component of skin, bone, tendon, and other fibrous connective tissues.

성분

All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type I collagen differs from other collagens by its low lysine hydroxylation and low carbohydrate composition.

제조 메모

Prepared by a modification of the pepsin extraction and salt fractionation method of Niyibizi, C., et al., J. Biol. Chem., 259, 14170 (1984). It is an acid soluble collagen that can be dissolved in water with acetic acid added to pH 3.0 (5 mg/mL), yielding an opalescent, colorless solution.

기타 정보

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Be wary of confusing Sigma-type designations with recognized collagen classification types.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)