C9281

Cholesterol Esterase from Pseudomonas fluorescens

lyophilized powder, ≥10,000 units/g protein

• 동의어(들): CE, bile salt-stimulated lipase, cholesteryl ester hydrolase, pancreatic cholesterol esterase, Sterol-ester acylhydrolase
• CAS Number: 9026-00-0
• EC 번호: 3.1.1.13 (BRENDA, IUBMB)
• EC Number: 232-808-6
• MDL number: MFCD00071052
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: Pseudomonas fluorescens
• Quality Level: 200
• 분석: 10-30% (TCA-Biuret)
• 양식: lyophilized powder
• 특이 활성도: ≥10,000 units/g protein
• 분자량: ~129 kDa
• 구성: Protein, ~20%
• 저장 조건: under inert gas (argon)
• 기술: cell based assay: suitable
• 색상: tan to brown
• pH: 7-9
• solubility: 0.4 M potassium phosphate, pH 7.0: soluble 1.0 mg/mL
• 저장 온도: −20°C

설명

일반 설명

Research area: Cell Signaling

Cholesterol Esterase (CE) is a glycoprotein that can be isolated from fungal species such as Candida cylindracea and Pseudomonas fluorescens. It is classified as a member of the lipase/esterase family and functions as a homo-dimeric protein. CE is produced in the pancreas and is released in an active form upon stimulation by Cholecystokinin (CCK).

애플리케이션

Cholesterol esterase from Pseudomonas fluorescens has been used in:

• in cholesterol esterase assay to quantify total cholesterol from human blood serum samples
• a study to investigate the nondenaturing protein electro transfer of the esterase activity of lipolytic preparations
• an optimization study of components in enzymatic cholesterol reagents containing cholesterol oxidase
• for the modification of human plasma low-density-lipoprotein (LDL) to induce endothelial cell (EC) dysfunction and monocyte (MC) adhesion in the branched tissue-engineered blood vessels (TEBVs)
• to hydrolyze native cholesterol ester (CE) during ​filipin staining for detection of CE within the retinal frozen sections

This enzyme is widely used in the determination of serum cholesterol in diagnostic laboratories.

생화학적/생리학적 작용

Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols. This activity of CE plays a vital role in steroid biosynthesis or de novo steroidogenesis. Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramides. The enzyme may have multiple functions in lipid and lipoprotein metabolism, as well as in atherosclerosis..

기타 정보

Contains potassium phosphate and TRITON® X-100.

단위 정의

One unit will hydrolyze 1.0 μmole of cholesteryl oleate to cholesterol and oleic acid per min at pH 7.0 at 37 °C in the presence of taurocholate.

분석 메모

Protein determined by biuret.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)