CS0340

Dihydrofolate Reductase Assay Kit

1 kit sufficient for 50-100 tests

• 동의어(들): DHFR Assay Kit
• UNSPSC 코드: 12161503
• NACRES: NA.84

속성

• Quality Level: 200
• 사용: kit sufficient for 50-100 tests
• 배송 상태: dry ice
• 저장 온도: −20°C
• 유전자 정보: human ... DHFR(1719)

설명

애플리케이션

The kit is used for the detection of DHFR activity and screening of DHFR inhibitors. The assay is based on the ability of DHFR to catalyze the reversible NADPH-dependent reduction of dihydrofolic acid to tetrahydrofolic acid. The reaction progress is followed by monitoring the decrease in absorbance at 340 nm.
Dihydrofolic acid+NADPH+H⁺ ↔ Tetrahydrofolic acid+NADP⁺

생화학적/생리학적 작용

DHFR (dihydrofolate reductase) is an enzyme that catalyzes a reaction essential for the biosynthesis of nucleotidic bases of DNA. Blocking the enzyme causes cell death as a result of DNA synthesis inhibition. DHFR is an excellent target for anti-tumor drugs.

특징 및 장점

• Quick and simple method.
• The kit contains all the reagents required for a colorimetric assay of DHFR activity in cell lysates, tissue homogenates, or column fractions of purified enzyme.
• The kit includes a purified enzyme for use as a positive control and screening of DHFR inhibitors.
• The kit includes methotrexate (MTX), a prokaryotic and eukaryotic DHFR specific inhibitor, which exhibits anti-tumor activities.
• The kit was tested on A431, NIH-3T3, and CHO cell lines, rat liver, kidney, brain, and skeletal muscle tissue extracts, and recombinant DHFR.

키트 구성품 전용

• Assay Buffer 10x for DHFR 30 mL
• Dihydrofolate Reductase (DHFR) human .1 U
• Dihydrofolic acid (DHFR substrate) 3 x 10
• Amethopterin (+)(methotrexate, MTX)
  (DHFR inhibitor) 2 x 10
• NADPH (β-Nicotinamide adenine dinucleotide phosphate reduced tetrasodium salt) 25 mg

안전성 정보

• 픽토그램: GHS06,GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H301,H315,H319,H335,H360FD
• 예방조치 성명서: P202 - P261 - P264 - P301 + P310 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Oral - Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable