E0157

Anti-eIF2α antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• 동의어(들): Anti-EIF2, Anti-EIF2S1, Anti-Eukaryotic translation initiation factor 2, subunit 1, Anti-eIF-2A
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: IgG fraction of antiserum
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 양식: buffered aqueous solution
• 분자량: antigen ~35 kDa
• 종 반응성: human
• 기술: immunohistochemistry: 1:250 using human cerebral cortex sections ; western blot: 1:250-1:500 using PC-12 cell lysate
• UniProt 수납 번호: P05198
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... EIF2S1(1965)

설명

일반 설명

The eukaryotic translation initiation factor 2 subunit α (EIF2A) gene is mapped to human chromosome 14q23.3. The eukaryotic translation initiation factor 2 (eIF2), a multimeric protein is composed of three subunits termed α, β, and γ.

특이성

Anti-eIF2α (also known as eIF-2A) recognizes human eIF2α.

애플리케이션

Anti-eIF2α antibody produced in rabbit has been used in western blotting.
Anti-elF2α antibody is also suitable for immunohistochemistry at a working dilution of 1:250 using human cerebral cortex sections.

Anti-eIF2α antibody produced in rabbit is suitable for immunoblotting at a working dilution of 1:250-1:500 using PC-12 cell lysate. It was used as a primary antibody to detect eIF2α in a study.

생화학적/생리학적 작용

Increased eIF2α phosphorylation has been observed in Alzheimer′s disease. Viral infections are also known to induce eIF2α phosphorylation.

The main role of eIF2 complex is to transfer Met-tRNAi in the presence of GTP to the 40S ribosomal subunit during the initiation of translation. Regulation of the initiation of translation at the level of eIF2 involves the phosphorylation of eIF2α at a conserved Ser⁵¹ residue. This leads to inhibition of eIF2β activity, thus reducing guanine nucleotide exchange and consequently, the translation rate. Protein synthesis inhibition at the level of eIF2α phosphorylation is linked to a variety of cell stress signaling pathways via specific kinases. Phosphorylation of eIF2α reduces the rate of translation and gives the cell time to correct the stress damage, and selectively enhance the translation of genes important for stress remedy.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

저장 및 안정성

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
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