F0628

Ferredoxin-NADP⁺ Reductase from Spinacia oleracea (spinach)

lyophilized powder, ≥15 units/mg solid, secondary activity: ≥10 units/mg solid NADPH diaphorase

• 동의어(들): Ferridoxin-NADP⁺ oxidoreductase
• CAS Number: 9029-33-8
• EC 번호: 1.18.1.2 (BRENDA, IUBMB)
• MDL number: MFCD00131050
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 양식: lyophilized powder
• Quality Level: 200
• 특이 활성도: ≥15 units/mg solid
• 외래 활성: NADH diaphorase activity ≤10 units/mg solid
• 저장 온도: −20°C

설명

일반 설명

Ferredoxin-NADP⁺ Reductase is a ferredoxin-soluble partner with a molar ratio to PSI of 0.9 and 3.

애플리케이션

Ferredoxin-NADP⁺ Reductase from Spinacia oleracea (spinach) has been used to test the effect of potential protein partners on the activity of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH)−CP12 preparation. It has also been used in standard assay conditions for phycocyanobilin:ferredoxin oxidoreductase (PcyA) enzyme assay.

Ferredoxin-NADP⁺ Reductase was used in in vitro ferredoxin-dependent desaturation of fatty acids in cyanobacterial thylakoid membranes. It was also used to regulate glyceraldehyde-3-phosphate dehydrogenase.

생화학적/생리학적 작용

Ferredoxin-NADP⁺ reductase catalyzes the reversible conversion of reduced ferredoxin to oxidized ferredoxin during photosynthesis. Ferredoxin-NADP(H) reductase constitutes a family of hydrophilic FAD-containing monomeric enzymes that deliver NADPH or low potential one-electron donors to redox-based metabolisms in plastids, mitochondria, and bacteria.

Ferredoxin-NADP⁺ reductase catalyzes the reversible conversion of reduced ferredoxin to oxidized ferredoxin during photosynthesis. Ferredoxin-NADP(H) reductase constitutes a family of hydrophilic FAD-containing monomeric enzymes that deliver NADPH or low potential one-electron donors to redox-based metabolisms in plastids, mitochondria, and bacteria.

단위 정의

One unit will reduce 1.0 millimole of cytochrome C per min at pH 7.5 at 25 °C in the presence of spinach ferredoxin and NADP.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)