추천 제품
Product Name
Granulocyte colony-stimulating factor human, G-CSF, recombinant, expressed in E. coli, suitable for cell culture
생물학적 소스
human
Quality Level
재조합
expressed in E. coli
분석
≥95% (SDS-PAGE)
양식
lyophilized powder
효능
0.01-0.1 ng/mL
품질
endotoxin tested
분자량
18.8 kDa (175 amino acids including N-terminal methionine)
포장
pkg of 5 and 25 μg
기술
cell culture | mammalian: suitable
불순물
<1 EU/μg (LAL test)
UniProt 수납 번호
저장 온도
−20°C
유전자 정보
human ... CSF3(1440)
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일반 설명
Granulocyte colony-stimulating factor human (GCSF) gene is mapped to human chromosome 17q21.1. Structurally, GCSF comprises a four α-helical bundle.
애플리케이션
Granulocyte colony-stimulating factor human has been used:
- in culturing human neutrophils
- as a growth factor component of double layer granulocyte-macrophage colony-forming unit (CFU-GM) culture system for CD34+ cells
- to test its protective effects chronic liver damage model
생화학적/생리학적 작용
Granulocyte colony-stimulating factor human (GCSF) interacts specifically with the immunoglobin (Ig) domain of the granulocyte colony-stimulating factor receptor (GCSFR).
Granulocyte-colony stimulating factor (G-CSF) is a proliferation, differentiation, survival, and activation factor for hematopoietic cells of the restricted neutrophilic granulocyte lineage. It is produced by macrophages activated by endotoxin (LPS), by monocytes activated by TNF-α with IFNγ, by fibroblasts and endothelial cells activated by IL-1 or TNF-α, and by bone marrow stromal cells activated by IL-1 or LPS. It is suggested that during the inflammatory process endotoxins stimulate tissue macrophages to produce not only G-CSF but several other cytokines, including IL-1 and TNF-α, which in turn stimulate more G-CSF release from endothelial cells and fibroblasts. G-CSF can also synergize with IL-3 to shorten the G0 period of early hematopoietic progenitors. In addition to the namesake proliferative activity, G-CSF acts on mature neutrophils to enhance their survival and to stimulate their tumorcidal activity. Human G-CSF binds and activates a 130 kD to150 kD glycoprotein single chain receptor that has been classified as a member of the hematopoietic (cytokine) receptor family, the cytokine receptor class I, or the gp130 related cytokine receptor family (although it does not apparently bind to gp130). G-CSF receptors can be found on neutrophils, myeloid leukemia cells that respond to G-CSF, bone marrow cells of neutrophilic granulocyte lineage, and on placental trophoblasts, plus a soluble form may be expressed. Two forms of human G-CSF (177 and 174 amino acids) are synthesized from a single gene by alternative splicing, but murine G-CSF is a single expressed form of 178 amino acids. Human and murine G-CSF share 73% amino acid sequence homology and full cross-reactivity.
물리적 형태
Lyophilized from 0.2 μm-filtered solution of 10 mM HOAc containing 50 μg BSA per μg G-CSF
분석 메모
The biological activity is measured in a cell proliferation assay using a murine myeloblastic cell line, NFS-60.
관련 제품
제품 번호
설명
가격
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
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