G0774

Glycerokinase from Bacillus stearothermophilus

buffered aqueous solution, ≥75 units/mg protein (biuret)

• 동의어(들): ATP:glycerol 3-phosphotransferase, Glycerol Kinase
• CAS Number: 9030-66-4
• EC 번호: 2.7.1.30 (BRENDA, IUBMB)
• EC Number: 232-862-0
• MDL number: MFCD00131205
• UNSPSC 코드: 12352204
• eCl@ss: 32160410
• NACRES: NA.54

속성

• 생물학적 소스: Bacillus sp. (Bacillus steaarothermophilus)
• Quality Level: 200
• 분석: ≥5.0 mg protein/mL (biuret)
• 양식: buffered aqueous solution
• 특이 활성도: ≥75 units/mg protein (biuret)
• 저장 조건: dry at room temperature
• 색상: beige
• 응용 분야: life science and biopharma
• 저장 온도: 2-8°C
• 유전자 정보: Bacillus sp. ... glpK(89613566)

설명

일반 설명

Research area: Cell Signaling

Glycerol kinase is encoded by the GK gene on chromosome Xp21.2. Glycerol kinase is predominantly active in the liver.

애플리케이션

Glycerokinase from Bacillus stearothermophilus has been used in steady-state kinetics studies.

생화학적/생리학적 작용

Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. It is also subject to feedback regulation by fructose-1,6-bisphosphate.Oxidized glycerol-3-phosphate results in dihydroxyacetone phosphate which enters either glycolysis or gluconeogenesis. Elevated glycerol is observed in glycerol kinase deficiency leading to pseudohypertriglyceridemia.

단위 정의

One unit will convert 1.0 μmole of glycerol and ATP to L-α-glycerophosphate and ADP per min at pH 9.8 at 25 °C in a coupled system with PK/LDH.

물리적 형태

Stabilized solution in Tris buffer, pH 7.3

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)