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Merck
모든 사진(1)

주요 문서

F0137

Sigma-Aldrich

Fructose-6-phosphate Kinase from Bacillus stearothermophilus

Type VII, lyophilized powder, ≥50 units/mg protein

동의어(들):

6-Phosphofructokinase, ATP:D-fructose 6-phosphate 1-phosphotransferase

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About This Item

CAS Number:
EC 번호:
EC Number:
MDL number:
UNSPSC 코드:
12352204
NACRES:
NA.54

유형

Type VII

Quality Level

양식

lyophilized powder

특이 활성도

≥50 units/mg protein

분자량

34 kDa

배송 상태

wet ice

저장 온도

−20°C

관련 카테고리

일반 설명

Fructose-6-phosphate kinase from Bacillus stearothermophilus, a 34 kDa enzyme, belongs to phosphofructokinase (PFK) - like superfamily. The glutamate 161 and arginine 162 residues at the active site are crucial for substrate binding.
Research Area: Cell Signaling
Bacillus stearothermophilus
phosphofructokinase (BsPFK) is a homotetramer that is allosterically inhibited by phosphoenolpyruvate (PEP), which binds along one dimer-dimer interface.

애플리케이션

Fructose-6-phosphate Kinase from Bacillus stearothermophilus was shown to interact with neuronal nitric oxide synthase (nNOS) causing a defect in glycolytic metabolism and increased fatigability in dystrophic muscle.
Fructose-6-phosphate Kinase from Bacillus stearothermophilus has been used in the assay mixture for mass spectrometry assay for phosphoglucoisomerase (PGI) (G6P to F6P reaction).
Fructose-6-phosphate kinase from Bacillus stearothermophilus has been used:
  • in steady state analysis of phosphofructokinase activity in the presence of ATP deuterated at the C8 position(C8-D ATP)
  • for standard curve generation for quantifying muscle phosphofructokinase (PFK) activity

생화학적/생리학적 작용

Fructose-1,6-bisphosphatase (FBP) is an important enzyme in glucose metabolism. It catalyzes the hydrolysis of fructose-1,6-bisphosphate to fructose-6-phosphate and inorganic phosphate. Fructose-6-phosphate kinase converts fructose-6-phosphate into fructose 1,6-bisphophate in the rate limiting step of the glycolysis cycle.
Phosphofructokinase (PFK) is an essential bifunctional enzyme that serves as a critical regulator in the intermediate stages of glycolysis. PFK is strongly linked to caveolae and is brought to caveolae by caveolin-1 in vascular smooth muscle cells (VSMCs).

단위 정의

One unit will convert 1.0 μmole of fructose 6-phosphate and ATP to fructose 1,6-diphosphate and ADP per minute at pH 9.0 at 30 °C.

물리적 형태

Lyophilized powder containing buffer salt (e.g. phosphate buffer, or Tris buffer with NaCl)

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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